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関連する概念動画

Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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関連する実験動画

Updated: Jun 21, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

RNA:誘導遺伝子サイレンス

M Matzke1, A J Matzke, J M Kooter

  • 1Institute of Molecular Biology, Austrian Academy of Sciences, A-5020 Salzburg, Austria. mmatzke@imb.oeaw.ac.at

Science (New York, N.Y.)
|August 11, 2001
PubMed
まとめ

二重鎖RNAの小さなRNAは,様々な生物の遺伝子サイレンシングを誘発する. このRNAサイレンシングメカニズムは,植物や動物における遺伝子発現に,転写後および表遺伝的に影響を及ぼします.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • エピジェネティクス エピジェネティクス

背景:

  • 小型RNAは遺伝子発現の重要な調節体である.
  • RNAサイレンシングは,様々な生物で保存されている生物学的プロセスです.
  • 二重鎖RNA (dsRNA) は,小さなRNAの生成の前駆体である.

研究 の 目的:

  • 小型RNA媒介遺伝子サイレンシングのメカニズムを解明する.
  • エピジェネティック・レギュレーションにおけるRNAサイレンシングの役割を強調する.
  • 開発におけるRNAサイレンシングの重要性を探求する.

主な方法:

  • dsRNA分裂による小RNAバイオゲネシスの分析.
  • サイトプラズマとゲノムの遺伝子サイレンシング経路の調査.
  • mRNAの分解による転写後の遺伝子静止を研究する.
  • 植物におけるDNAメチル化による転写遺伝子の静止を検証する.

主要な成果:

  • 小型のRNAは,細胞質とゲノムで表遺伝子遺伝子の静止を誘導する.
  • 小型RNAは,補完的なメッセンジャーRNAの分解を誘導する.

さらに関連する動画

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
09:18

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

Published on: June 2, 2016

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

関連する実験動画

Last Updated: Jun 21, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
09:18

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

Published on: June 2, 2016

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

  • 植物は,DNAメチル化による転写遺伝子サイレンシングのためにRNAサイレンシングを使用します.

    • 結論:

    • RNAサイレンシングは,外来配列に対する強力な防御です.
    • 小型RNA経路は,植物や動物の発達において重要な役割を果たします.
    • エピジェネティック・モディフィケーションは,RNA媒介の遺伝子調節の中心的なものです.