ホックス遺伝子の局所的で一時的な転写は,パターニングとセグメンテーションクロックとの間のリンクを示唆しています
1Department of Zoology and Animal Biology, University of Geneva, Sciences III, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.
Cell
|August 21, 2001
まとめ
発達中の胚におけるホックス遺伝子の活性化は,ダイナミックなストライプで発生し,セグメンテーションと調整されます. このプロセスは,ノッチシグナル伝達に依存し,遺伝子発現をセグメンテーションクロックとリンクします.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝子調節 遺伝子調節
- 分子生物学は分子生物学である.
背景:
- 発達中のホックス遺伝子の転写は染色体順に続いて,プレソミティック・メソデルマで発生する.
- ホックス遺伝子の活性化とソミトゲネシスを調整する正確なメカニズムは不明である.
研究 の 目的:
- プレソミティック・メソデルマにおけるホックス遺伝子発現の空間時間的動態を調査する.
- ソミトゲネシス中のホックス遺伝子活性化を調節するNotchシグナル伝達の役割を明らかにする.
主な方法:
- マウス胚におけるホックス遺伝子発現パターン (Hoxd1等) の分析.
- 機能的なNotch経路エフェクタが欠けているRBPJk欠乏性のマウスにおけるHox遺伝子発現の調査.
主要な成果:
- ホックス遺伝子は,プレソミト性メソダーマの内部で,ダイナミックな表現のストライプを示します.
- 転写突起は,このダイナミックな発現の基礎であり,潜在的にホックス活性化とソミトゲネシスを調整します.
- RBPJk欠乏したマウスは,プレソミト性メソデームにおけるHoxd遺伝子発現が著しく低下し,Notchシグナル伝達が示唆されている.
結論:
- ホックス遺伝子の活性化とセグメンテーションクロックの間には,ノッチシグナル伝達によって媒介される分子リンクが存在する.
- このリンクは,胚のセグメントの同期された生産と形態学的仕様を保証します.
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