ホモジゴス型ホモシスティヌリアにおける酸化ストレスと血小板活性化
G Davì1, G Di Minno, A Coppola
1Center of Excellence on Aging, Department of Biomedical Sciences, University of Chieti G. D'Annunzio, Chieti, Italy. gdavi@unich.it
Circulation
|September 6, 2001
まとめ
重度の高血糖糖血症 (CbetaSD) は酸化損傷を増加させ,血小板活性化を高めます. ビタミンEのサプリメントは,患者のこの損傷と活性化のマーカーを減少させた.
科学分野:
- バイオケミストリー バイオケミストリー
- 心血管医学 心血管医学
- メタボリック障害 メタボリック障害
背景:
- シスタチオニンベータ合成酵素 (CbetaSD) 欠乏によって引き起こされる重度の高血糖血症は,早発性アテロトロンボ性血管疾患と関連しています.
- ホモシステインは,酸化ストレスメカニズムによる血管損傷に関与しています.
研究 の 目的:
- CbetaSD患者において,8-イソプロスタグランジンF ((2α) のインビボ形成が上昇しているかどうかを調査する.
- この上昇が活体内の血小板活性化と相関するかどうかを判断するために,血小板糖代謝産物の排泄によって測定します.
- これらのマーカーに対するビタミンEサプリメントの効果を評価する.
主な方法:
- 13人の同卵性CbetaSD患者と健康な対照群から尿と血液のサンプルを採取した.
- 測定された尿中の8-イソ-PGF (((2α) と11-デヒドロ-TXB (((2) の排泄.
- 血ホモシステインとビタミンEのレベルを評価した.
- 投与されたビタミンE (600 mg/日2週間) と再測定されたマーカー.
主要な成果:
- CbetaSD患者では,対照群と比較して,尿中の8-iso-PGF(2alpha) と11-dehydro-TXB(2) の分泌量が著しく高かった.
- 両方のマーカーは,血のホモシステインと相互に相関していました.
- ビタミンEのサプリメントは,血のビタミンE濃度を著しく上昇させ,8-イソ-PGF (α) と11-デヒドロ-TXB (β) の排泄を減少させた.
- 尿中の8-イソ-PGFと血のビタミンE濃度の間に強い逆相関が観察されました.
結論:
- アラキドン酸のF(2) -イソプロスタンへの強化された過酸化は,CbetaSDにおけるハイパーホモシステネミアと血小板活性化を結びつける可能性があります.
- これらの発見は,CbetaSD患者に対するビタミンEサプリメントのさらなる調査を支持します.
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