関連する実験動画
Updated: Jul 16, 2026
05:52
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
Published on: November 19, 2009
電圧によって引き起こされる膜の動き
P C Zhang1, A M Keleshian, F Sachs
1HHMI Center for Single Molecule Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Nature
|September 28, 2001
まとめ
膜間電圧は,膜の緊張を変化させることで細胞の動きを駆動する. 細胞の硬さと表面電荷の影響を受けるこの電気運動性は,HEK293細胞で原子力顕微鏡を用いて確認されました.
科学分野:
- バイオフィジックス 生物物理学
- 細胞電気生理学 細胞電気生理学
背景:
- 熱力学は,トランスメブラン電圧が膜張力に影響し,細胞の動きにつながると予測しています.
- 細胞の硬さと表面電位が,この運動の方向と大きさを決定する.
研究 の 目的:
- 電圧による細胞移動の熱力学的予測を実験的に確認する.
- 電圧,セル特性,運動の関係を定量化するために.
- 表面電荷の役割と,電動性に対するサリシラートの効果を調査する.
主な方法:
- 原子力顕微鏡 (AFM) を利用して,電圧で締め付けられたHEK293細胞の動きを追跡しました.
- 異なった強度のイオン溶液で実験を行った.
- 表面積密度を推定するために物理モデルを適用した.
主要な成果:
- HEK293 セルにおける電圧誘発のセル移動 (電動性) が確認されました.
- 普通の塩溶液での外向きの動きと,低イオン強度溶液での内向きの動きを観察した.
- 運動振幅は電圧 (~1 nm/100 mV) とインデント深さと相関する.
- 外部と内部表面の積荷密度が推定されている.
- サリシラートは,外部電荷を増加させることで電動性を阻害し,プレスティンの役割の再評価の可能性を示唆しています.
結論:
- 実験的証拠は,トランスメブラン電圧駆動の細胞移動の熱力学的予測を裏付けている.
- 細胞の電気移動性は,イオン強度,電圧,細胞の機械的性質に依存しています.
- 表面電荷は電動性を調節する上で重要な役割を果たし,プレスティンなどの機械感受性タンパク質に影響を及ぼします.
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Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
01:24Resting Membrane Potential
The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
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The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
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