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Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
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ドロソフィラのPDGF/VEGF受容体による細胞移動の誘導
P Duchek1, K Somogyi, G Jékely
1European Molecular Biology Laboratory, Developmental Biology Programme, 69117 Heidelberg, Germany.
Cell
|October 12, 2001
まとめ
ドロソフィラのオオゲネシスにおける指向された細胞移動には,EGFRと冗長なPVRシグナル伝達が含まれています. RacとそのアクティベーターであるMBCは,開発にとって極めて重要なこのガイドラインを仲介しています.
科学分野:
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- 導かれた細胞移動は,胚の発達にとって極めて重要です.
- 細胞の移動を導く分子メカニズムを in vivo で理解することは依然として課題です.
- ドロソフィラ・オオゲネシス (Drosophila oogenesis) 中の境界細胞の移動は,扱えるモデルシステムを提供しています.
研究 の 目的:
- ドロソフィラ・オオゲネシスにおける指向細胞移動を制御するシグナル伝達経路と分子機構を調査する.
- 境界細胞の誘導に関与する主要な受容体と下流エフェクターを特定する.
主な方法:
- ドロソフィラのオオゲネシスをモデルシステムとして利用した.
- 境界細胞移動における受容体チロシンキナーゼPVRの役割を調査した.
- PVRのリガンドとしてのPDGF/VEGF因子1 (PVF1) の機能を調べました.
- PVRと表皮成長因子受容体 (EGFR) 信号伝達の相互作用を評価した.
- Racとその活性化剤 Mbc/DOCK180/CED-5.の関与を分析した.
主要な成果:
- PVRは受容体チロシンキナーゼであり,境界細胞を卵細胞に導くのに不可欠です.
- 卵細胞はPVRのリガンドであるPVF1を分泌する.
- PVR媒介のガイドラインは,EGFRシグナル伝達とほぼ冗長である.
- RacとRacアクティベーター Mbc/DOCK180/CED-5はPVR誘導信号のメディエーターとして作用する.
結論:
- PVRシグナル伝達は,ドロソフィラオオゲネシス (Drosophila oogenesis) の間,指向された細胞移動において重要な役割を果たします.
- PVRとEGFRの冗長性は,細胞誘導における複雑な信号ネットワークを強調しています.
- RacとMbc/DOCK180/CED-5は,PVRを媒介する誘導経路の重要な下流コンポーネントである.
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