UAP56とAlyの間の直接的な相互作用によってリンクされたプレ-mRNAスプライシングとmRNAエクスポート
1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature
|October 26, 2001
まとめ
スプライシングファクターUAP56は,mRNAエクスポートファクターAlyをスプライシングされたmRNA-タンパク質複合体に誘導する. この相互作用は,mRNAスプライシングとエクスポートをコップリングする上で極めて重要です.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- メッセンジャーRNA (mRNA) のスプライシングとエクスポートは,コップリングされたプロセスである.
- mRNAの輸出因子Alyは,輸出のためのスプライスされたmRNA-タンパク質複合体 (mRNP) に採用されます.
- mRNPへのAly採用のメカニズムは不明である.
研究 の 目的:
- スプライスされたmRNP.へのAly採用のメカニズムを調査する.
- クーリング mRNA スプライシングとエクスポートに関与する要因を特定する.
主な方法:
- UAP56とAly結合を評価するためのタンパク質相互作用研究.
- mRNP内のUAP56とAlyの局所化の分析.
- 支配的-否定的阻害およびサイト指向型変異生成を用いた機能的測定法.
主要な成果:
- DEAD-boxヘリカーゼUAP56は,特にAly.と相互作用する.
- UAP56とAlyは,スプライスされたmRNPで共局所されています.
- 過剰なUAP56はmRNAの輸出とAlyの採用を阻害する.
- UAP56の相互作用を妨害するAlyの突然変異は,Alyの採用を妨げます.
結論:
- UAP56は,結合されたmRNPにAlyを勧誘する上で重要な役割を果たしています.
- UAP56は,スプライシングと輸出機械の架け橋として機能します.
- この採用は,効率的なmRNA輸出に不可欠です.
関連する概念動画
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...