スプライシング因子Sub2pは,Yari1pとの相互作用によって核mRNAの輸出に必要である
1BZH--Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Nature
|October 26, 2001
まとめ
スプライソソームタンパク質Sub2pは,酵母におけるmRNAエクスポートに不可欠であり,Yara1pと相互作用して輸送を促進します. この相互作用は,Yari1pをmRNAに誘導し,その核輸出を可能にするために鍵となる.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- イースト核タンパク質 Yra1pは,メッセンジャーRNA (mRNA) の輸出に不可欠である.
- Yra1pは,核孔にあるmRNA輸送因子Mex67p/Mtr2pと相互作用する.
研究 の 目的:
- スプライシングに関与するDEADボックスヘリケーズであるSUB2のmRNAエクスポートにおける役割を調査する.
- mRNA輸出の文脈で Yra1p と Sub2p の間の相互作用を解明する.
主な方法:
- YRA1 と SUB2.2 の間の遺伝的相互作用の分析
- タンパク質とタンパク質の相互作用を研究するために,in vivoおよびin vitro結合測定法.
- タンパク質結合ドメインの分析と競合アッセイ.
主要な成果:
- SUB2の変異または過剰発現はmRNAの輸出を阻害する.
- Yra1pとSub2pは,体内および体外の両方で直接結合する.
- Sub2pとMex67p/Mtr2pは,Yari1pで同じドメインへのバインディングを競っている.
結論:
- スプライソソームの成分Sub2pはmRNAの輸出に重要な役割を果たしています.
- Sub2pは,輸出のためのmRNAにYara1pを勧誘する可能性がある.
- Mex67p/Mtr2pがYara1pと結合すると,Sub2pが移動し,核孔通過が容易になる可能性があります.
関連する概念動画
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...


