Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Alveolar Abscess.

The Dental register·2021
Same author

Opening the Bite with Cap-Fillings, Preserving the Vitality of the Pulp.

The Dental register·2021
Same author

Alveolar Abscess.

The American journal of dental science·2019
Same author

The ionic layer is required for efficient dissociation of the SNARE complex by alpha-SNAP and NSF.

Proceedings of the National Academy of Sciences of the United States of America·2002
Same author

The Di-leucine motif of vesicle-associated membrane protein 4 is required for its localization and AP-1 binding.

The Journal of biological chemistry·2001
Same author

Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and syntaxin 1a.

The Journal of biological chemistry·2001

関連する実験動画

Updated: Jul 11, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

細胞生物学. 細胞生物学について. SNAREsなしの融合とは何か?

S J Scales1, M F Finley, R H Scheller

  • 1Genentech Inc., South San Francisco, CA 94080, USA. sscales@gene.com

Science (New York, N.Y.)
|November 3, 2001
PubMed
まとめ

分子の再編成は膜融合を推進し,神経伝達物質の放出などのプロセスに不可欠です. スケール et al. この複雑な細胞現象を制御するタンパク質の相互作用に関する新しい発見をレビューします.

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞神経科学 細胞神経科学とは

背景:

  • 膜融合は,シナプスで神経伝達物質の放出を含む細胞機能に不可欠です.
  • 膜融合を媒介するタンパク質機構を理解することは,細胞生物学における重要な課題です.

研究 の 目的:

  • 最近の研究をレビューするために (Schoch et al., Wang et al.) 膜融合におけるタンパク質の相互作用について.
  • 膜融合の分子メカニズムについての洞察を提供するため.

主な方法:

  • 出版された研究のレビュー.
  • 膜融合に関連するタンパク質相互作用データの分析.

主要な成果:

  • 膜融合に関与するタンパク質の相互作用に新たな光が照らされる.
  • 核融合中の分子再配置の詳細な議論.

結論:

  • レビューされた研究は,膜融合を理解する上で重要な進歩をもたらしています.
  • タンパク質のダイナミクスに関するさらなる研究は,シナプス機能に関する知識を高めるでしょう.

さらに関連する動画

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10:58

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
07:30

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Published on: December 8, 2019

関連する実験動画

Last Updated: Jul 11, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10:58

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
07:30

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Published on: December 8, 2019