ポテトのアーバスキュラを含む細胞で表現されたリン酸輸送物質
1Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Experimental Station Eschikon 33, 8315 Lindau, Switzerland.
Nature
|November 24, 2001
まとめ
植物の土地の植民に不可欠なアーバスキュラー菌根の共生は,栄養素の交換を伴う. 研究者らは,ポテトのStPT3リン酸トランスポーター遺伝子を特定し,この重要な植物と真菌のパートナーシップにおけるその役割を明らかにしました.
科学分野:
- 植物生物学 植物生物学
- 菌類学 菌類学とは
- 遺伝学 遺伝学とは
背景:
- 茂み状菌根は,一般的な植物根の共生であり,土地の植民に不可欠である.
- この相互の共生は,植物がリンを,真菌がグルコースを摂取することで,栄養素の交換を容易にする.
- 状菌糸体共生の基礎となる分子メカニズムは,依然としてほとんど解明されていない.
研究 の 目的:
- ポテトの状菌根共生に関与する重要な遺伝子を特定する.
- リン酸輸送遺伝子StPT3.3の機能と発現パターンを調査する.
主な方法:
- 酵母補充の測定は,タンパク質の機能性を確認するために行われます.
- 遺伝子発現パターンを決定するためにRNAの局所化とリポーター遺伝子発現分析を行います.
- StPT3プロモーター地域のバイオ情報分析.
主要な成果:
- リン酸トランスポーター遺伝子StPT3は,ジャガイモ (Solanum tuberosum) で特定されました.
- StPT3タンパク質の機能性は,酵母補充によって確認されました.
- 菌根構造を形成するジャガイモの根領域でStPT3発現が観察されました.
- StPT3のプロモーターモチーフは,共生進化における遺伝的再編成の役割を示唆しています.
結論:
- StPT3遺伝子は,ジャガイモの状菌根共生に役割を果たしています.
- StPT3プロモーターの遺伝的再編成が,この相互共生の進化に寄与した可能性がある.
関連する概念動画
Short-distance Transport of Resources
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
ABC Transporters: Exporter
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
ABC Transporters: Importer
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Aquaporins
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
Carrier-Mediated Transport
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...


