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関連する概念動画

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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関連する実験動画

Updated: Jul 15, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

バクテリアの複製フォークにある2つの必須DNAポリメラーゼ.

E Dervyn1, C Suski, R Daniel

  • 1Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, 78352 Cedex, France.

Science (New York, N.Y.)
|November 27, 2001
PubMed
まとめ

バシルス・サブティリスは,複製のために1つではなく,2つの重要なDNAポリメラーゼを使用します. 新しく特定されたdnaE(BS) 遺伝子は,polCと共に,バクテリアのDNA合成と細胞活力にとって極めて重要です.

科学分野:

  • 微生物学 微生物学とは
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • バクテリアのDNA複製には,伝統的に単一のDNAポリメラーゼが不可欠であり,その例はE. coliのdnaEとB. subtilisのpolCである.
  • E. coli の dnaE と同型である B. subtilis での dnaE (((BS) の発見は,その役割に関するさらなる調査を促した.

研究 の 目的:

  • バシルス・サブティリスのDNA複製におけるdnaE(BS) 遺伝子の本質と機能を調査する.
  • B. subtilisが複製フォークで1つ以上のエッセンシャルDNAポリメラーゼを持っているかどうかを判断する.

主な方法:

  • バクテリア遺伝学とゲノム分析.
  • B. subtilis. の細胞生存能力とDNA複製延長を評価する.
  • レイギングストランド合成における dnaE (BS) の役割と,複製工場との関連を調査する.

主要な成果:

  • B. subtilis の dnaE(BS) 遺伝子は,polC.に類似して,細胞の生存能力とDNA複製の延長に不可欠です.
  • B. subtilisは,複製フォークで2つの異なるDNAポリメラーゼを有しています.
  • dnaE (BS) は,遅滞性鎖の合成に関与し,複製工場と関連しています.

さらに関連する動画

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
04:25

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

Published on: June 13, 2025

関連する実験動画

Last Updated: Jul 15, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
04:25

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

Published on: June 13, 2025

結論:

  • バシルス・サブティリスは,複製のために2つの必須のDNAポリメラーゼを使用しており,これは以前,真核生物で観察された発見です.
  • これは,2つのポリメラーゼ (polCとdnaE) がB. subtilisや他のバクテリアの両方のDNA鎖の合成を扱う保存されたメカニズムを示唆しています.