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関連する概念動画

pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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関連する実験動画

Updated: Jun 28, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

細胞生物学. 細胞生物学について. mRNAの輸出へのタッピング

M J Moore1, M Rosbash

  • 1Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA. mmoore@brandeis.edu

Science (New York, N.Y.)
|December 1, 2001
PubMed
まとめ
この要約は機械生成です。

研究者らは,ペプチド阻害剤を用いた新しい方法を開発し,生細胞の核から細胞質への伝達 RNA (mRNA) 輸出経路を追跡した.

さらに関連する動画

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
10:26

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Published on: September 10, 2015

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10:56

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Published on: January 16, 2018

関連する実験動画

Last Updated: Jun 28, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
10:26

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Published on: September 10, 2015

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10:56

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Published on: January 16, 2018

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • 核から細胞質へのメッセンジャーRNA (mRNA) 輸出は,遺伝子発現に極めて重要です.
  • 個々のmRNAの特定の輸出経路を特定することは,分子生物学における大きな課題となっています.

研究 の 目的:

  • mRNAの輸出経路を調査するための新しい戦略を提示する.
  • 生体細胞におけるmRNA輸送メカニズムの研究を可能にする.

主な方法:

  • 細胞に浸透するペプチド阻害剤を用いた新しいアプローチの議論.
  • これらの阻害剤を適用して,特定のmRNA輸出経路を解明する.

主要な成果:

  • 提案された戦略は,複雑なmRNAの輸出ルートを解剖する新しい方法を提供します.
  • 生体細胞内のmRNA輸送ダイナミクスのリアルタイム分析を可能にします.

結論:

  • この革新的な方法は,mRNA核輸出を理解するための強力なツールを提供します.
  • 輸出アダプタとレセプタの役割に関する詳細な調査を容易にする.