バクテリアファージの細胞壁のヒドロラーゼによって,Streptococcus pneumoniaeを迅速に殺す
J M Loeffler1, D Nelson, V A Fischetti
1Laboratory of Bacterial Pathogenesis, The Rockefeller University, New York, NY 10021, USA.
まとめ
新しい酵素であるPalは,耐性菌株を含む肺炎性ストレプトコッカスを鼻から迅速に除去する. この酵素は高い特異性を示し,耐性を防止し,肺炎球菌の伝播に対する新たな介入の可能性を提供します.
科学分野:
- 微生物学 微生物学とは
- バイオテクノロジー バイオテクノロジー
- 感染症 感染症は感染症です.
背景:
- Streptococcus pneumoniaeの鼻内伝播は,コミュニティでの伝播のための重要な貯蔵庫です.
- 肺炎球菌の伝播を排除するための現在の介入は,特に抗生物質耐性菌株については,限られている.
研究 の 目的:
- 浄化された肺炎球菌菌ファージリチス酵素 (Pal) が,ストレプトコッカス肺炎伝染菌を迅速に排除する効果を評価する.
- 酵素の特異性と耐性開発の可能性を評価する.
主な方法:
- ペニシリン耐性菌株を含む15種類の一般的なStreptococcus pneumoniae血清型に対するPal酵素の試験.
- 肺炎球菌のコロニゼーションのマウスモデルを用いたインビボ研究で,酵素の有効性とバクテリアの位数への影響を評価した.
- パル酵素が他の喉頭微生物に及ぼす影響の評価と,パル耐性肺炎球菌の評価.
主要な成果:
- パル酵素は,接触から数秒以内に15種類の一般的なStreptococcus pneumoniae血清型の迅速な殺戮を実証しました.
- In vivoでは,単一のPal酵素治療により,以前にコロニー化されたマウスは5時間後に検知できない肺炎球菌の位数で表されました.
- この酵素は,他の口頭の微生物にほとんど影響を及ぼせず,長時間曝露後にPal耐性肺炎球菌は検出されなかった.
結論:
- Purified Pal酵素は,Streptococcus pneumoniaeを駆除する非常に効果的で迅速な薬剤です.
- Pal酵素は優れた特異性を発揮し,共生植物に害を与えることなく,耐性を誘発することなく,pneumococciを標的にします.
- Palは,その貯蔵庫をターゲットにすることで,肺炎球菌病の制御のための有望な新しい治療候補を表しています.
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