CTCFは,X不活性化選択のトランス作用因子候補である
Wendy Chao1, Khanh D Huynh, Rebecca J Spencer
1Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
Science (New York, N.Y.)
|December 18, 2001
まとめ
この研究では,哺乳類のX不活性化中のX染色体選択の重要な要因としてCTCFを特定しています. Tsix遺伝子内のCTCF結合部位は,どのX染色体が静止されるかを調節するエピジェネティックスイッチを形成します.
科学分野:
- エピジェネティクス エピジェネティクス
- ゲノミクスゲノミクスとは
- 哺乳類の遺伝学 哺乳類の遺伝学
背景:
- X不活性化 (X-inactivation) は,雌性哺乳類における重要なプロセスであり,X染色体の2つのうちの1つを静止させます.
- このプロセスは,非コーディングXist (無活性X特異トランスクリプト) 遺伝子によって調節され,Tsix遺伝子によって対抗されます.
- Tsix内の選択/インプリントセンターは,X染色体の選択に役割を果たします.
研究 の 目的:
- X不活性化時にX染色体選択に関与するトランス作用因子を特定する.
- Tsix遺伝子の選択/インプリントセンターにおけるCTCFの役割を調査する.
主な方法:
- CTCF結合部位の機能をテストするために増強剤阻害アッセイを使用しました.
- CTCF結合に対するCpGおよび非CpGメチレーションの効果を研究した.
- Tsixにおける選択/インプリントセンターの削除がX染色体選択に与える影響を分析した.
主要な成果:
- X染色体選択の候補として,断熱剤と転写因子であるCTCFを特定しました.
- 選択/インプリントセンターには,強化剤阻害アッセイで機能するタンドムCTCF結合部位が含まれていることが実証されました.
- CTCF結合はCpGメチル化に敏感であり,非CpGメチル化によって廃止されていることが示されました.
結論:
- CTCFとTixは,X無活性化のための規制された表遺伝子スイッチを確立するために協力しています.
- CTCF結合部位におけるメチル化パターンは,X染色体の選択を制御する可能性がある.
さらに関連する動画
12:42Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Published on: November 26, 2014
08:27A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
Published on: May 22, 2019
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