ミトコンドリア結合因子6は,人間の血管内皮細胞の表面に存在し,剪定ストレスによって放出されます
1Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan. osanait@cc.hirosaki-u.ac.jp
Circulation
|December 19, 2001
まとめ
血管内皮細胞は,プレッサー物質であるミトコンドリア結合因子6 (CF6) を放出する. 切断ストレスは,これらの細胞からCF6の放出を大幅に増加させ,循環中のCF6の調節を示します.
科学分野:
- 心血管生物学 心血管生物学
- 細胞生理学 細胞生理学
- バイオケミストリー バイオケミストリー
背景:
- ミトコンドリア結合因子6 (CF6) は,プロスタサイクリン合成の内生性阻害剤である.
- CF6は全身循環で循環し,ラットでは圧力物質として作用します.
- 循環中のCF6の起源は,血管内皮細胞に焦点を当てて調査されました.
研究 の 目的:
- 血管内皮細胞が循環するCF6の源であるかどうかを判断する.
- 内皮細胞からCF6の放出に流体切断ストレスが及ぼす影響を調査する.
主な方法:
- 培養ヒト静脈内皮細胞 (HUVEC) とECV 304細胞を使用した.
- 表面に関連したCF6を検出するための免疫光顕微鏡.
- 静的および切断ストレス条件下における培養媒体のCF6濃度測定.
- 細胞ホモゲネートとミトコンドリアのCF6含有量を分析した.
- 評価されたCF6とGAPDH mRNAの比率.
- 表面関連CF6.6を定量化するためのフローサイトメトリ.
主要な成果:
- 免疫光検査は,ECV 304とHUVECの両方の表面関連CF6を確認しました.
- 静的条件下では,媒体のCF6濃度が時間とともに増加した.
- 流体シアストレスはCF6の放出を大幅に強化し,シアレベルが高くなった場合 (25ダイネ/cm2) 濃度が劇的に上昇しました.
- 切断ストレスにより,細胞ホモゲネートとミトコンドリアのCF6含有量が15ダイネ/cm2で増加した.
- シーアストレスは,CF6とGAPDH mRNAの比率を上昇させた.
- 表面に関連したCF6は,シアストレスの曝露後に増加した.
結論:
- 血管内皮細胞は,CF6の重要な源である.
- 内皮細胞から表面に関連したCF6の放出は,流体剪定ストレスによって調節されます.
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