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関連する概念動画

Titration of a Polyprotic Acid02:08

Titration of a Polyprotic Acid

A polyprotic acid contains more than one ionizable hydrogen and undergoes a stepwise ionization process. If the acid dissociation constants of the ionizable protons differ sufficiently from each other, then the titration curve for such polyprotic acid generates a distinct equivalence point for each of its ionizable hydrogens. Therefore, titration of a diprotic acid results in the formation of two equivalence points, whereas the titration of a triprotic acid results in the formation of three...
Acid-Base Titration Curves02:23

Acid-Base Titration Curves

A titration curve is a plot of some solution property versus the amount of added titrant. For acid-base titrations, solution pH is a useful property to monitor because it varies predictably with the solution composition and, therefore, may be used to monitor the titration’s progress and detect its endpoint. Acid-base titration can be performed with a strong acid and a strong base, a strong acid and a weak base, or a strong base and a weak acid.
For a titration carried out for 25.00 mL of 0.100...
Solution Composition During Acid/Base Titrations01:17

Solution Composition During Acid/Base Titrations

The titration of a weak acid with a strong base results in the formation of water and the conjugate base of the acid. For instance, titrating acetic acid with sodium hydroxide leads to the formation of water and sodium acetate. A solution of acetic acid and sodium acetate constitutes a buffer whose relative concentration at different stages of the titration is indicated by the α values, which represent percentages of the weak acid and its conjugate base.
The α0 and α1 values represent the...
Titration of Polyprotic Base with a Strong Acid01:18

Titration of Polyprotic Base with a Strong Acid

The titration of a polyprotic base such as sodium carbonate with a strong acid such as hydrochloric acid results in two equivalence points on the titration curve. At the first equivalence point, the carbonate ions in the base are completely converted to bicarbonate ions. The second equivalence point corresponds to the complete conversion of bicarbonate ions to carbonic acid, which dissociates into carbon dioxide and water. The region before the first equivalence point corresponds to the...
Titration of a Weak Acid with a Strong Base01:30

Titration of a Weak Acid with a Strong Base

In titrating a weak acid with a strong base, different calculation methods are applied at various stages. Initially, the pH of a weak acid like acetic acid is calculated using its dissociation constant (Ka) and an ICE table. Upon addition of a strong base such as sodium hydroxide, a buffer forms, and its pH is determined using the Henderson-Hasselbalch equation. As more base is added and the titration reaches the halfway point, the pH becomes equal to the pKa of the acid, indicating equal...
Titration of Polyprotic Acids with a Strong Base01:23

Titration of Polyprotic Acids with a Strong Base

Titration of a polyprotic acid, which contains multiple ionizable protons, involves distinct dissociation steps, each with its own dissociation constant (Ka). Each successive Ka is weaker than the previous one. In the titration of a polyprotic acid like sulfurous acid with a strong base such as sodium hydroxide, the base first neutralizes the initial ionizable proton, forming an intermediate species (e.g., hydrogen sulfite ions). This step's titration curve resembles that of a weak monoprotic...

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関連する実験動画

Updated: Jul 5, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

マルチサイトリン酸化とS相までのカウントダウン.

R J Deshaies1, J E Ferrell

  • 1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. deshaies@its.caltech.edu

Cell
|January 10, 2002
PubMed
まとめ
この要約は機械生成です。

SCF (Cdc4) ユビキチンリガゼは,Sic1を5ではなく6つのリン酸でターゲットにしています. この発見は,アナログ信号をタンパク質ナノプロセッサのデジタル出力に変換する分子カウントメカニズムを明らかにしています.

さらに関連する動画

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte
10:27

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte

Published on: October 5, 2017

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

関連する実験動画

Last Updated: Jul 5, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte
10:27

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte

Published on: October 5, 2017

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

科学分野:

  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー
  • セルラーレギュレーション セルラーレギュレーション

背景:

  • Sic1は,リン酸化によって調節される細胞サイクル阻害剤です.
  • SCF (Cdc4) は,細胞サイクル進行に関与するE3ユビキチンリガゼです.
  • リン酸化レベルはしばしばタンパク質の機能と分解を左右する.

研究 の 目的:

  • Sic1のユビキチネーションについてSCF ((Cdc4) によって認識された正確なリン酸化値を調べる.
  • SCF (Cdc4) がSic1の異なるリン酸化状態を区別するメカニズムを解明する.
  • アナログのリン酸化信号が生物学的文脈でどのようにデジタル出力に変換されるかを理解する.

主な方法:

  • 精製されたSCF (((Cdc4) とSic1の変種を用いたインビトロ結合測定では,定義されたリン酸化状態が示されています.
  • ユビキチネーションアッセイは,SCFによってSic1の改変をモニタリングする (Cdc4).
  • リン酸化部位を確認するための質量スペクトロメトリーと,Sic1.のステキオメトリー.

主要な成果:

  • SCF ((Cdc4) は6箇所で酸化されたSic1を選択的に結合し,5箇所で酸化しない.
  • このリン酸化に依存するユビキチネーションは,Sic1の分解を誘発する.
  • この研究では",アナログ"入力 (リン酸塩の数) に基づく明確な"デジタル"スイッチが示されています.

結論:

  • SCF ((Cdc4) は分子カウンターとして作用し,基板の認識のために正確なリン酸化値を使用します.
  • このメカニズムは,アナログの入力 (さまざまな酸化レベル) からデジタル出力 (ubiquitination/degradation) を提供します.
  • このタンパク質ナノプロセッサのアーキテクチャを理解することで,細胞の正確な調節と信号伝達に関する洞察が得られます.