関連する実験動画
Updated: May 3, 2026

09:40
In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
Published on: November 2, 2017
6.6K
ヌクレオポリンRanBP2は,SUMO1 E3リガゼの活性性を有する
Andrea Pichler1, Andreas Gast, Jacob S Seeler
1Max-Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany.
Cell
|January 17, 2002
まとめ
ヌクレオポリンRanBP2/Nup358はSUMO1 E3のような活性を示し,Ubc9酵素と相互作用することによってSUMOylationを強化します. この発見は,タンパク質の改変と,NPCでの核輸入を関連付けています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- SUMOylationによる翻訳後の改変は,相互作用,局所化,および安定性を含む主要なタンパク質機能を調節する.
- SUMOylation経路は,E1酵素 (Aos1/Uba2) とE2酵素 (Ubc9) を含み,PIASタンパク質はE3のような因子として識別されています.
- 核孔複合体 (NPC) は,核と細胞質の間の輸送を調節するために不可欠です.
研究 の 目的:
- ヌクレオポリンRanBP2/Nup358.8の潜在的SUMO E3のような活性について調査する.
- RanBP2/Nup358のSUMO E3のような活動に起因するメカニズムと特定のドメインを特徴付ける.
- 核輸入に関連するRanBP2/Nup358媒介SUMOylationの機能的影響を決定する.
主な方法:
- SUMO1 転送強化を評価するための生化学的測定法.
- RanBP2/Nup358とUbc9.9との相互作用を特定するためのタンパク質相互作用研究
- RanBP2/Nup358.8内のSUMO E3のような活動領域のマッピング
- NPCでのSUMOylation活動の局所化研究.
主要な成果:
- RanBP2/Nup358は,PIASタンパク質とRING指のモチーフから独立して,SUMO1 E3のような活性を示しています.
- RanBP2/Nup358は,E2酵素Ubc9.9と直接相互作用する.
- E3のような活動は,RanBP2/Nup358.8.の特定の33 kDa領域内に存在する.
- RanBP2/Nup358によって媒介されるSUMOylationの活動は,NPCの細胞質フィラメントに局限しています.
結論:
- RanBP2/Nup358は,新しいSUMO1 E3型のリガゼとして機能します.
- この研究は,ニュクレオポリンによって媒介されるSUMOylationと核輸入プロセスとの間の直接的なリンクを特定しています.
- これらの発見は,タンパク質の改変と輸送がNPCで協調されたイベントであることを示唆しています.
関連する概念動画
The Nucleolus
8.6K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.6K
Nuclear Protein Sorting
4.8K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.8K
Nuclear Localization Signals and Import
6.2K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
6.2K
Nuclear Export
3.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.7K
Directionality of Nuclear Transport
3.9K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.9K
Regulation of Nuclear Protein Sorting
2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K

