セパラーゼ,ポロキナーゼ,キネトコアタンパク質Slk19,およびSpo12は,初期のアナフェーズ中にCdc14の局所化を制御するネットワークで機能します
Frank Stegmeier1, Rosella Visintin, Angelika Amon
1Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
Cell
|February 8, 2002
まとめ
FEARネットワークは,芽生えた酵母における初期アナフェーズ中に,その阻害剤からCdc14の放出を開始します. この早期の放出は,継続的なCdc14活性のためにミトスの脱出ネットワーク (MEN) を刺激します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- Cdc14フォスファテーゼは,芽生えた酵母菌におけるミトス脱出の重要な調節剤である.
- Cdc14は,その阻害体であるCfi1/Net1によって,アナフェーズまで核olusに隔離されます.
- ミトスの脱出ネットワーク (MEN) のシグナリングカスケードは,Cdc14の放出を制御する.
研究 の 目的:
- Cdc14の早期リリースを制御する新しい規制ネットワークを特定し,特徴づけること.
- FEARネットワークと MEN経路の相互作用を解明する.
主な方法:
- 芽生えた酵母 (Saccharomyces cerevisiae) の遺伝子解析について.
- 細胞サイクル内のタンパク質の相互作用と調節作用を調査する.
- 主要なタンパク質にフォーカスする:Cdc14,Cfi1/Net1,Cdc5,Esp1,Slk19,Spo12.
主要な成果:
- Cdc5,Esp1,Slk19,Spo12を含むFEAR (Cdc14早期アナフェーズ放出) ネットワークの識別.
- FEARネットワークが初期アナフェーズ中にCfi1/Net1からCdc14の放出を促進することを実証.
- MEN経路が,後期アナフェーズ中にCdc14の放出を維持している証拠.
結論:
- FEARネットワークは,MENの上流でCdc14の放出を開始します.
- FEARネットワークによって放出されたCdc14は,MENの活動を刺激し,ポジティブなフィードバックループを作成する可能性があります.
- この協調的な行動は,ミトーシスからの適時かつ規制された脱出を保証します.
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