関連する実験動画
Updated: Jul 11, 2026

09:40
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
Published on: February 13, 2012
C. elegans の全身性RNAiには,推定されるトランスメブランタンパク質SID-1が必要である.
William M Winston1, Christina Molodowitch, Craig P Hunter
1Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
まとめ
C. elegans の二重鎖RNA干渉 (RNAi) は,遺伝子サイレンシング情報を細胞間で拡散させます. SID-1タンパク質は,この全身的なRNAi伝播に不可欠であり,細胞自律的に作用する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 発達生物学 発達生物学について
背景:
- 双鎖RNA媒介遺伝子干渉 (RNAi) は,糸虫Cayenorhabditis elegansの遺伝子発現を抑制する強力なツールです.
- システミックRNAiは,遺伝子サイレンシングを組織全体に拡散させることができますが,細胞間の情報伝送のメカニズムは不明です.
研究 の 目的:
- C. elegans.におけるRNAiサイレンシング信号の全身伝播の基礎となる細胞および分子メカニズムを調査する.
- RNAi効果の細胞間拡散に関与する遺伝的位置を特定する.
主な方法:
- 系統的なRNAiを視覚化するためのC. elegans株の構築.
- 系統的なRNA干渉欠陥 (サイド) 変異体を特定するための遺伝子スクリーニング.
- 特定された遺伝子の分子特徴付け,遺伝子クローンとタンパク質の局所化研究を含む.
主要な成果:
- RNAi.の全身的拡散に必要ないくつかのサイドロシの特定.
- sid-1ロクスは,保存されたトランスメブランタンパク質,SID-1をコードする.
- SID-1はRNAi感受性細胞で発現し,細胞周辺に局所化し,細胞自律的に機能し,全身RNAiを媒介する.
結論:
- SID-1タンパク質は,C. elegans.における全身性RNAiの間に,遺伝子静止情報の細胞間伝達において重要な役割を果たしています.
- SID-1は,組織間でのRNAi効果の拡散に責任を負う経路の重要な構成要素を表しています.
関連する概念動画
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

