隣接するゾーンの化学吸引剤に対するバランスの取れた反応は,B細胞の位置を決定する

Karin Reif¹, Eric H Ekland, Lars Ohl

¹Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143-0414, USA.

Nature

|March 8, 2002

まとめ

抗原に結合したBリンパ球は,CCR7.7を上調することでTゾーンに移動する. このケモカイン受容体は,抗体反応と免疫細胞の相互作用に不可欠な,彼らの移住を誘導します.

科学分野:

免疫学免疫学とは
細胞生物学細胞生物学
分子生物学は分子生物学である.

背景:

B型リンパ球は,二次リンパ性臓器で循環し,抗原を探します.
抗原に結合したB細胞は,抗体反応に不可欠なTゾーン境界のTヘルパー細胞と相互作用する.
抗原結合後のB細胞のTゾーンへの移住を駆動するメカニズムは未定義のままである.

研究の目的:

抗原の結合後にB細胞がTゾーンに移転する分子メカニズムを解明する.
抗原主導のB細胞移動に関与する重要なケモカイン受容体とリガンドを特定する.

主な方法:

抗原に結合したB細胞におけるケモカイン受容体発現 (CCR7,CXCR5) の分析.
特定のケモカイン (CCL19,CCL21) またはB細胞受容体 (CCR7) を欠いているノックアウトマウスを用いた研究.
レトロウイルス媒介による遺伝子転送は,細胞移動を誘導するCCR7およびCXCR5発現の十分性を評価する.

主要な成果:

抗原の関与は,TゾーンケモカインCCL19とCCL21の受容体であるCCR7のB細胞発現を増加させます.
抗原との接触時にB細胞がTゾーンに移動することは,CCL19/CCL21キモカインとCCR7発現に依存しています.
CCR7の発現の増加は,B細胞をTゾーンに誘導するのに十分であり,CXCR5過剰発現は,この移動を覆すことができます.

結論:

ケモカイン受容体反応のバランス (CCR7とCXCR5) は,リンパ性臓器内のB細胞の位置づけを決定する.
抗原結合時にCCR7のアップレギュレーションが,B細胞のTゾーンへの移住を促す主なメカニズムである.
この研究は,抗原誘発B細胞の移転の分子基礎を定義し,免疫反応の理解に影響を与えています.

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