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関連する概念動画

Photosystem II01:22

Photosystem II

The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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関連する実験動画

Updated: Jul 5, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

RNAポリメラーゼII機構:構造が機能を明るくする

Nancy A Woychik1, Michael Hampsey

  • 1Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.

Cell
|March 23, 2002
PubMed
まとめ

ユカリオットの転写は,RNAポリメラーゼII,初期因子,およびメディエーター複合体に依存する. 構造的な洞察,特に酵母RNAポリメラーゼIIに関する洞察は,転写機構に光を当てます.

科学分野:

  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは
  • バイオケミストリー バイオケミストリー

背景:

  • ユカリオットの転写は,基本的な生物学的プロセスです.
  • キープレーヤーは,RNAポリメラーゼII,開始因子,そしてメディエーター複合体を含む.
  • 転写メカニズムを理解することは,細胞機能にとって極めて重要です.

研究 の 目的:

  • ユカリオットの転写のメカニズムを解明する.
  • 転写因子や複合体に関する構造的な洞察を提供するためです.
  • イーストRNAポリメラーゼIIの原子解像度構造に注目する.

主な方法:

  • トランスクリプションコンポーネントの3次元構造決定.
  • 高解像度構造分析. 高解像度構造分析. 高解像度構造分析. 高解像度構造分析.
  • イーストRNAポリメラーゼIIの比較構造研究.

主要な成果:

  • 複数の転写因子や複合体の構造データも得られた.
  • 詳細な3次元構造は,分子相互作用の洞察を提供します.
  • 酵母RNAポリメラーゼIIの原子解像度構造が決定されました.

さらに関連する動画

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

関連する実験動画

Last Updated: Jul 5, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

結論:

  • 構造生物学は,真核細胞の転写に関する重要な洞察を提供します.
  • メディエーター・コンプレックスは,規制信号の伝達において重要な役割を果たします.
  • 酵母RNAポリメラーゼII構造は,転写開始の詳細なモデルを提供します.