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Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Transcription01:10

Transcription

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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Transcription01:17

Transcription

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Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Transcription01:17

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関連する実験動画

Updated: Apr 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

14.2K

mRNA処理と転写を統合する.

Nick J Proudfoot1, Andre Furger, Michael J Dye

  • 1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom. nicholas.proudfoot@path.ox.ac.uk

Cell
|March 23, 2002
PubMed
まとめ
この要約は機械生成です。

キャピング,スプライシング,ポリアデニレーションなどのメッセンジャーRNA (mRNA) 処理イベントは,転写中に起こります. これらのプロセスは,転写によって調整され,互いに影響し合う.

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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Last Updated: Apr 6, 2026

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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝子発現の表現について
  • RNA 処理 RNA 処理

背景:

  • キャピング,スプライシング,ポリアデニレーションを含むメッセンジャーRNA (mRNA) 処理は,遺伝子発現における重要なステップです.
  • これらの処理イベントは,遺伝子の転写の間に発生することが知られている.
  • これらの反応の連携と相互作用は完全に理解されていません.

研究 の 目的:

  • mRNA処理のコトランスクリプションの性質を明らかにする.
  • カッピング,スプライシング,およびポリアデニレーションの間の調整を調査する.
  • これらの統合されたプロセスを促進するRNAポリメラーゼIIの役割を理解する.

主な方法:

  • コトランスクリプションのmRNA処理経路の分析.
  • 転写とmRNA処理因子の間の分子相互作用を調査する.
  • RNAポリメラーゼIIのリン酸化C末端ドメイン (CTD) の機能を研究する.

主要な成果:

  • mRNAのキャピング,スプライシング,ポリアデニレーションは,転写と同時に行われます.
  • これらの処理反応は,効率と特異性に対して相互に影響を及ぼします.
  • トランスクリプションは,これらのmRNA処理イベントを積極的に調整します.
  • RNAポリメラーゼIIのリン酸化CTDは,処理因子との主要な分子相互作用のプラットフォームとして機能します.

結論:

  • mRNA処理は,統合されたコトランスクリプションプロセスです.
  • トランスクリプションは,mRNAのキャッピング,スプライシング,ポリアデニレーションの調整に中心的な役割を果たします.
  • RNAポリメラーゼIIのCTDは,トランスクリプションとmRNAの成熟を結びつけるのに不可欠です.