関連する実験動画
Updated: May 10, 2026

10:12
Visualization of G3BP Stress Granules Dynamics in Live Primary Cells
Published on: May 21, 2014
Shaggy/GSK3は,Cubitus interruptusを調節することにより,ヘッジホッグのシグナル伝達に敵対する
Jianhang Jia1, Kazuhito Amanai, Gelin Wang
1Center for Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9133, USA.
Nature
|March 26, 2002
まとめ
GSK3キナーゼであるShaggy (Sgg) タンパク質は,Ciタンパク質処理を促進することによって,ドロソフィラのヘッジホッグ経路を否定的に調節する. これは,ヘッジホッグのシグナル伝達におけるSgg/GSK3の新たな役割を明らかにしています.
科学分野:
- 発達生物学 発達生物学について
- 分子生物学は分子生物学である.
- 細胞シグナル伝達 細胞信号伝達
背景:
- シャギー (Sgg) とグリコゲン合成キナーゼ3 (GSK3) は,ウィングレス (Wg) とWnt経路の阻害剤として知られています.
- ヘッジホッグ (Hh) 経路は,胚の発達とパターニングに不可欠です.
研究 の 目的:
- ドロソフィラのヘッジホッグ (Hh) 信号伝達経路におけるShaggy (Sgg) の役割を調査する.
- Hh経路内のCubitus interruptus (Ci) タンパク質をSggが調節するメカニズムを解明する.
主な方法:
- ドロソフィラのSgg機能喪失変異体の遺伝子解析.
- Ciタンパク質の加工とリン酸化状態の調査.
- Hh反応性遺伝子発現の分析,デカペンタプレジック (dpp) およびwgを含む.
主要な成果:
- sgg機能の喪失は,完全な長さのCi (Ci155) の細胞自律的蓄積とHh反応性遺伝子の子宮外発現につながります.
- Sgg/GSK3は,PKAと併用して,Ciをハイパーフォスホルリ化し,それを抑制剤形態 (Ci75) に処理するためにターゲットにします.
- ヘッジホッグのシグナリングは,おそらくその脱酸化を促進することによって,Ciプロテオリシスに反対します.
結論:
- Shaggy (Sgg) /GSK3は,ドロソフィラのヘッジホッグ経路の負の調節剤として作用する.
- Sgg/GSK3とPKAは,Hh信号伝達における重要なイベントであるCiタンパク質処理を制御するために協力しています.
- ヘッジホッグシグナリングは,経路の活性を調節するために,Ciのリン酸化状態を調節します.
さらに関連する動画
関連する概念動画
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

