スーパーオキシド生成とノックス族のタンパク質の発現は,ヒトの大動脈硬化症における
Dan Sorescu1, Daiana Weiss, Bernard Lassègue
1Department of Medicine, Emory University, Atlanta, Ga 30322, USA.
Circulation
|March 27, 2002
まとめ
gp91phoxとNox4を含むNAD(P) H酸化酵素は,動脈硬化症における酸化ストレスに寄与する. これらのタンパク質は,ヒトの冠動脈内の特定の細胞に局所され,疾患の進行に影響を与えます.
科学分野:
- 心血管生物学 心血管生物学
- 酸化ストレス研究 酸化ストレス研究
- 動脈硬化症の病原性 病原性
背景:
- NAD(P) Hオキシダゼは,血管系でスーパーオキシドを生成する.
- 動脈硬化症のリスク因子と関連した酵素活性.
研究 の 目的:
- 超酸化物生産の局所を調査する.
- 人間の冠動脈におけるノックス族のタンパク質発現 (gp91phox,Nox1,Nox4) を決定する.
- 非動脈硬化性動脈と動脈硬化性動脈の表現を比較する.
主な方法:
- ヒトの冠動脈のセグメントでダイヒドロエチジウムを用いた細胞内超酸化物産生を調査した.
- ノックスタンパク質 (gp91phox,Nox1,Nox4) とp22phox.の評価された発現と局所化.
- 動脈硬化症の重度と細胞含有量 (マクロファージ,アルファ-アクチン陽性細胞) と相関するタンパク質発現.
主要な成果:
- スーパーオキシドは,非動脈硬化性動脈で均一に見られる.
- 動脈硬化性動脈のプラーク肩に局限した強い超酸化物.
- gp91phoxとp22phoxは主にマクロファージで,Nox4はノンファゴサイト細胞で,Nox1の発現は低かった.
- gp91phox/p22phox mRNAは動脈硬化症の重度と相関する; gp91phoxはマクロファージを含む; Nox4はアルファ-アクチン陽性細胞を持つ.
結論:
- gp91phoxとNox4は,動脈硬化症における酸化ストレスに寄与する.
- 細胞特異的発現は,疾患の発生と進行における役割を示唆しています.
- ノックスタンパク質は,ヒトの冠動脈動脈硬化症に関与している.
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