PSD-95のパルミテートサイクルによって調節されるシナプス強度
Alaa El-Din El-Husseini1, Eric Schnell, Srikanth Dakoji
1Department of Physiology, University of California, San Francisco, CA 94143, USA.
Cell
|April 17, 2002
まとめ
PSD-95のパルミテートサイクルは,シナプス強さとAMPA受容体の活性を調節する. このダイナミックなプロセスは,シナプス性可塑性と受容体の内在化に不可欠であり,神経細胞のコミュニケーションに影響を与えます.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- AMPA型グルタミン酸受容体のダイナミック調節により,シナプス強度が制御されます.
- ポストシナプス密度タンパク質95 (PSD-95) は,シナプス可塑性および受容体トラフィックに関与しています.
- シナプスのAMPA受容体調節を制御するメカニズムは完全に理解されていません.
研究 の 目的:
- シナプス性可塑性におけるPSD-95のパルミトイレーションの役割を調査する.
- グルタミン酸受容体の活性がPSD-95パルミトイロ化にどのように影響するか判断する.
- AMPA受容体トラフィックとシナプス強度におけるPSD-95パルミテートサイクルの機能を明らかにする.
主な方法:
- シナプスにおけるPSD-95におけるパルミテートサイクルの識別.
- グルタミン酸受容体の活性によるPSD-95パルミトイロ化調節の評価.
- シナプスPSD-95クラスターとAMPA受容体に対するパルミトイレーションを阻害する効果の分析.
- AMPA受容体の内部化ダイナミクスの調査.
- PSD-95,スターガジン,AMPA受容体クラスタリングを研究するために,非ニューロンモデルシステムを利用.
主要な成果:
- PSD-95におけるパルミテート循環は,グルタミン酸受容体の活性によって調節される.
- パルミトイロ化の急性阻害はシナプスPSD-95を分散させ,シナプスAMPA受容体を減少させます.
- グルタミン酸媒介のAMPA受容体内化には,PSD-95デパルミトイレーションが必要です.
- PSD-95のパルミトイロ化は,非ニューロン系におけるPSD-95,スターガジン,AMPA受容体のクラスタリングを調節する.
結論:
- PSD-95のパルミテートサイクリングは,シナプス強度を調節する重要なメカニズムです.
- このプロセスは,AMPA受容体の密輸と内部化を含む,活動に依存したシナプス可塑性に関与しています.
- この発見は,シナプス機能のための新しい規制経路を強調しています.
さらに関連する動画
09:07Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
Published on: August 15, 2017
11.8K
08:28Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
Published on: March 29, 2020
6.1K
関連する概念動画
Long-term Potentiation
51.6K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
51.6K
Long-term Potentiation
2.7K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when...
Hebbian LTP
LTP can occur when...
2.7K
Chemical Synapses
9.5K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
9.5K
Chemical Synapses
10.9K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
10.9K
Postsynaptic Potential (PSP)
11.8K
Postsynaptic potential (PSP) refers to a change in the electrical potential of a neuron when neurotransmitters released by presynaptic neurons bind to postsynaptic receptors. This potential can either be excitatory, leading to depolarization and ultimately action potential generation, or inhibitory, leading to hyperpolarization and suppression of the postsynaptic neuron.
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
11.8K
Integration of Synaptic Events
6.4K
Synaptic integration mainly includes the summation of graded potentials. Graded potentials, regardless of their type, cause subtle alterations in membrane voltage, resulting in either depolarization or hyperpolarization. These incremental changes, when combined or summed, can propel the neuron toward its threshold. Consider, for example, a membrane experiencing a +15 mV shift, causing it to depolarize from -70 mV to -55 mV. In this scenario, graded potentials govern the membrane's ability to...
6.4K
