アルテミスは,V(D) J再結合でヘアピン開く活動のための狩りを終了しますか?

Mark S Schlissel¹

¹Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. mss@uclink4.berkeley.edu

Cell

|April 17, 2002

まとめ

DNA依存タンパク質キナーゼ複合体 (DNA-PK) とアルテミスタンパク質は,ヘアピンDNAを開く. この複合体は,免疫システムの発達における重要なプロセスであるV(D) J再結合に不可欠です.

科学分野:

分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー
免疫学免疫学とは

背景:

DNA依存タンパク質キナーゼ (DNA-PK) は,DNA修復経路における重要な酵素である.
アルテミスは,二重鎖DNA破裂修復に関与する,最近特定されたタンパク質です.
V(D) J再結合は,多様な抗体およびT細胞受容体レパートリーを生成するために不可欠です.

研究の目的:

アルテミスタンパク質がDNA-PK触媒サブユニット (DNA-PKcs) と複合されたときの機能を調査する.
ヘアピンDNA開口におけるアルテミス-DNA-PKcs複合体の役割を明らかにする.
V(D) J再結合におけるこの複合体の生理学的関連性を決定する.

主な方法:

バイオケミカルアッセイは,ヘアピンDNAの開口活性を in vitro で評価する.
V(D) J再結合における複合体の役割を裏付けるための遺伝分析.

主要な成果:

アルテミスの複合体とDNA-PKcsは,ヘアピンDNA分子を開く能力を実証しました.
遺伝的および生化学的証拠の両方が,この複合体のV(D) J再結合における役割を支持しています.

結論:

アルテミス-DNA-PKcs複合体は,ヘアピン開く活性を持っています.
この複合体は,おそらく,V(D) J再結合中のヘアピン開封に起因する生理学的活動である.

さらに関連する動画

06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

関連する概念動画

02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

02:31

Homologous Recombination

01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...

関連する実験動画