肝臓と心臓における広範囲で異なった昼夜性遺伝子発現
Kai-Florian Storch1, Ovidiu Lipan, Igor Leykin
1Department of Neurobiology, Harvard Medical School, Northeastern University, Boston, Massachusetts 02115, USA.
Nature
|April 23, 2002
まとめ
哺乳類の周辺組織は,肝臓と心臓の異なる毎日のリズムを持つ広範な昼夜遺伝子調節を示しています. 両方において調節される遺伝子はほとんどなく,重複する機能にもかかわらず,専門的な経路を示している.
科学分野:
- *クロノバイオロジー
- * 分子生物学について
- *生理学 *生理学
背景:
- *日経時計は,哺乳類の周辺組織における毎日の生理学的プロセスを調節する内生的な振動器である.
- * 異なる組織における昼夜間の遺伝子調節の範囲と特異性は,ほとんど不明のままである.
- * 組織特異的な昼間の経路を理解することは,生理学的タイミングメカニズムを解読する上で極めて重要です.
研究 の 目的:
- * マウスの肝臓と心臓におけるインビボ昼間遺伝子発現を比較的に分析する.
- *これらの周辺組織における昼夜遺伝子調節の範囲を決定する.
- * 組織間の昼間の経路の重複と分岐を調査する.
主な方法:
- *遺伝子発現分析には,12488の遺伝子を代表するオリゴヌクレオチド配列を使用した.
- *昼間の遺伝子発現の比較分析は,マウスの肝臓と心臓で行われました.
- * リズム遺伝子を特定するために,in vivo遺伝子発現パターンを研究した.
主要な成果:
- * 広範な昼夜遺伝子調節が観察され,各組織で発現する遺伝子の8-10%に影響を与えました.
- * 肝臓と心臓の間では,日経周期の分布が著しく異なることが判明しました.
- * 組織特異的な遺伝子発現に関係なく,両方の組織において,非常に少数の遺伝子 (最小の重複) が,日中時計の調節を示した.
結論:
- * 周回性昼夜遺伝子調節は広範囲にわたるが,組織特異性が高い.
- * 肝臓と心臓の異なる昼夜調節には,重複する,しかし多様な生物学的プロセスが含まれています.
- * 37の遺伝子のコアセットは,循環因子に反応する遺伝子を含め,潜在的な新しい時計と出力遺伝子を示唆しています.
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