リボヌクレオペプチド複合体を用いたATP結合受容体のインビトロ選択

Takashi Morii¹, Masaki Hagihara, Shin-ichi Sato

¹Institute of Advanced Energy, Kyoto University, and PRESTO, Japan Science and Technology Corporation, Uji, Kyoto 611-0011, Japan. t-morii@iae.kyoto-u.ac.jp

Journal of the American Chemical Society

|April 25, 2002

まとめ

研究者らは,ペプチドとRNAの支架を使用してATPのための新しいリボ核ペプチド受容体を設計した. この人工受容体は人工受容体です.

科学分野:

分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー
構造生物学構造生物学とは

背景:

リボソームは,RNA-タンパク質複合体の多様な構造を示しています.
人工受容体は,分子認識と薬剤設計において極めて重要です.

研究の目的:

ペプチド-RNA複合体を用いた新種の人工受容体を設計する.
アデノシン三リン酸 (ATP) に対する特定のリボヌクレオペプチド受容体を作る.

主な方法:

短いペプチドとRNAをランダム化された核酸領域と組み合わせた支架を設計した.
リボヌクレオペプチドプール形成のためにHIV-1 Rev応答要素を利用した.
特定の結合特性を有するRNAオリゴヌクレオチドを分離するために,in vitro選択で用いられる.

主要な成果:

ATPに特異性が高いリボヌクレオペプチド受容体を成功裏に生成しました.
特定されたATP結合リボヌクレオペプチド配列は,既知のATPアプタマーとは異なっていた.
ATP結合はRevペプチドの存在に依存し,そのアミノ酸置換によって調節された.

結論:

ペプチド成分は,RNA受容体の機能的構造に不可欠です.
ペプチドのRNA結合領域の外にあるアミノ酸は,ATP結合に影響する.
このアプローチは,カスタムリボヌクレオペプチド受容体および酵素を設計するための戦略を提供します.

さらに関連する動画

08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

08:31

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

Published on: June 30, 2021

関連する概念動画

01:55

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...

01:32

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...

01:31

ATP Energy Storage and Release

ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...

01:28

ATP and Macromolecule Synthesis

Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...

01:31

ATP Energy Storage and Release

01:32

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...

関連する実験動画

リボヌクレオペプチド複合体を用いたATP結合受容体のインビトロ選択

科学分野:

背景:

研究 の 目的:

主な方法:

主要な成果:

結論:

さらに関連する動画

関連する概念動画