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関連する概念動画

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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関連する実験動画

Updated: Jul 18, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

酵母におけるmRNA処理の全ゲノム分析は,スプライシング特有のマイクロアレイを用いて行われました.

Tyson A Clark1, Charles W Sugnet, Manuel Ares

  • 1Department of Molecular, Cell, and Developmental Biology, Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA.

Science (New York, N.Y.)
|May 4, 2002
PubMed
まとめ

研究者は,mRNA処理因子を研究するために,マイクロアレイを使用して酵母遺伝子のスプライシングをマッピングしました. Prp17p と Prp18p のようなキーファクターは,イントロンを,特に短距離のイントロンを除去するために不可欠です.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • イントロンとは,真核生物の遺伝子内の非コーディング配列である.
  • メッセンジャーRNA (mRNA) スプライシングはイントロンを除去して成熟したmRNAを形成します.
  • mRNAスプライシング中のゲノム解釈の正確なメカニズムは完全に理解されていません.

研究 の 目的:

  • スプライシングにおけるmRNA処理因子の全ゲノム規模の影響を調査する.
  • 異なるmRNA処理因子間の機能的関係を特定する.
  • mRNA処理に対する遺伝子特有の依存性を理解する.

主な方法:

  • スプライスされたRNAとスプライスされていないRNAを区別するためのカスタムマイクロアレイの開発.
  • 酵母における18のmRNA処理因子の枯渇時にスプライシングパターンの全ゲノム分析.
  • 遺伝子特異的インデックスを利用して,転写と衰退率を考慮します.
  • 定量ポリメラーゼ連鎖反応 (qPCR) を使用した配列の発見の確認.

主要な成果:

  • RNAスプライシングに対する共通の効果に基づいてmRNA処理因子間の機能的関係を特定した.

さらに関連する動画

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

関連する実験動画

Last Updated: Jul 18, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

  • 特定のmRNA処理因子に対する差異的な依存性を示す異なる遺伝子群が明らかになった.
  • Prp17pとPrp18pは,短距離のブランチポイントから3'のスプライスサイト距離を持つイントロンの除去に不可欠であることを確認しました.

    • 結論:

    • この研究は,酵母スプライシングにおけるmRNA処理因子機能の全ゲノム地図を提供します.
    • スプライスされたRNAと非スプライスされたRNAに共通する影響は,処理因子間の複雑な機能ネットワークを明らかにする.
    • 特定の因子であるPrp17pとPrp18pは,短いイントロンのスプライシングに不可欠な役割を果たし,メカニズム的な洞察を強調しています.