G0細胞のE2FおよびMyc反応性遺伝子を占有するクロマチンの修飾物質を含む複合体
Hidesato Ogawa1, Kei-Ichiro Ishiguro, Stefan Gaubatz
1Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA.
Science (New York, N.Y.)
|May 11, 2002
まとめ
E2F-6タンパク質複合体は,静止細胞におけるクロマチンの変形剤を介して遺伝子を静止させます. このメカニズムは,レチノブラストームのタンパク質に独立してヒストンの改変とポリコンブ群のタンパク質を含む.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- 細胞サイクル規制について
背景:
- E2F-6は,遺伝子サイレンシングに関与する転写因子です.
- その抑制メカニズムは,網膜芽細胞腫タンパク質とは独立しており,さらなる解明が必要である.
研究 の 目的:
- E2F-6タンパク質複合体を浄化し特徴づけるために.
- E2F-6媒介による遺伝子サイレンシングの基礎となる分子機構を理解するために.
主な方法:
- 培養細胞からE2F-6複合体の浄化.
- 関連するタンパク質とクロマチンの改変剤の識別.
- 異なる細胞サイクル段階における標的プロモーター占有率の分析.
主要な成果:
- E2F-6は,MagaとMaxと共にマルチメリック複合体を形成し,E2F,Myc,Brachyuryのサイトに結合する.
- この複合体には,新しいヒストンメチルトランスフェラーゼ (H3K9),HP1ガンマ,ポリコンブ群のタンパク質が含まれています.
- E2F-6複合体は,G1細胞よりもG0 (静止) 細胞のターゲットプロモーターを優先的に結合します.
結論:
- E2F-6複合体内のクロマチンの変形剤は,遺伝子サイレンシングを媒介する.
- この静止は静止細胞のE2FおよびMyc反応性遺伝子に影響する.
- E2F-6は,表遺伝的メカニズムを通して,細胞サイクル依存の遺伝子調節に重要な役割を果たします.
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