Cid13は,リボヌクレオチド還元酵素 (mRNA) を調節するサイトプラズミックポリア) ポリメラーゼです
Shigeaki Saitoh1, Andrei Chabes, W Hayes McDonald
1Department of Molecular Biology, The Scripts Research Institute, La Jolla, CA 92037, USA.
Cell
|June 14, 2002
まとめ
分裂酵母Cid13は, suc22 mRNAを特異的に標的にするサイトプラズマ的ポリ (A) ポリメラーゼです. この酵素は,ニュクレオチドプールを調節することによって,DNA複製とゲノム安定性の維持に不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 遺伝学 遺伝学とは
背景:
- 分裂酵母Cid13と芽生える酵母Trf4/5は,保存されたヌクレオチチルトランスフェラーゼ家族に属しています.
- 現在,Trf4/5は必須のDNAポリメラーゼと考えられています.
- 細胞内のCid13の正確な機能は,まだ完全に解明されていない.
研究 の 目的:
- 分裂酵母Cid13.の酵素活性と細胞の局所化を特徴づけること.
- Cid13.の特定のmRNA標的を決定する.
- DNA複製とゲノム維持におけるCid13の役割を調査する.
主な方法:
- ポリアデニレーション活性を決定する酵素測定法.
- 顕微鏡を用いた細胞の局所化に関する研究.
- dNTPプールのcid13変異体とヒドロキシウレア感受性の分析.
- 分子生物学技術を用いたmRNA標的の特定.
主要な成果:
- Cid13は,核ポリアデニラゼとは異なるポリアデニラゼとして機能します.
- Cid13はサイトプラズマに局所されています.
- Cid13は,リボヌクレオチド還元酵素 (RNR) サブユニットをコードする suc22 mRNA を特にポリアデニラートします.
- cid13変異体は,dNTPプールが減少し,ヒドロキシ尿素に対する感受性が低下しています.
結論:
- Cid13は,新しいサイトプラズマのポリ (A) ポリメラーゼを表しています.
- Cid13は,DNA複製に不可欠なヌクレオチドプールを調節する上で重要な役割を果たしています.
- Cid13は,RNRサブユニットmRNAを特異的に標的にすることで,ゲノム維持に重要である.
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