18SリボソームRNAバイオゲネシスに必要な大きな核状U3リボヌクレオプロテインです
François Dragon1, Jennifer E G Gallagher, Patricia A Compagnone-Post
1Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA.
Nature
|June 18, 2002
まとめ
研究者らは,大きなリボ核タンパク質複合体である小サブユニット (SSU) プロセソームを特定し,リボソームRNAの処理に不可欠である. この複合体には,U3小核核RNA (snoRNA) と28のタンパク質が含まれており,その中には18SrRNA生成に不可欠な17の新規成分が含まれています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- U3小核核RNA (snoRNA) は,エウカリオートにおけるリボソームRNA (rRNA) 処理に不可欠である.
- その機能と関連するタンパク質は,何十年もの間,ほとんど理解されていませんでした.
- U3スノRNAは,塩基配列相互作用を通じて,前-rRNAの分裂を媒介すると考えられている.
研究 の 目的:
- U3 snoRNAを含むリボヌクレオプロテイン (RNP) 複合体を浄化し特徴づけること.
- U3 snoRNA.に関連するタンパク質成分を特定するために.
- rRNA前処理におけるこの複合体の役割を明らかにする.
主な方法:
- 大量のRNP複合体をSaccharomyces cerevisiaeから浄化する.
- タンパク質の成分を特定するための質量スペクトロメトリ.
- 特定されたタンパク質の機能を評価するための枯渇研究.
主要な成果:
- U3スノRNAと28のタンパク質を含む大きな80S RNP複合体を浄化しました.
- 17種類の新しいタンパク質 (Utp1-17) とRrp5と,10種類の既知のタンパク質が同定されました.
- 新種のUtpタンパク質は核系であり,18SrRNAの生成に不可欠であり,活発なpre-rRNA処理複合体におけるその役割を示している.
- 複合体の大きさと機能からすると,小サブユニット (SSU) プロセソームと呼ばれる新生前リボソームRNAの末端ノブを表していることが示唆される.
結論:
- U3スノRNAは,プレ-rRNA処理のための大規模で不可欠な複合体の一部です.
- 特定されたSSUプロセソームは,18S.rRNAの生成に不可欠である.
- この発見は,リボソーム生体生成の基本的メカニズムに関する新しい洞察を提供します.
関連する概念動画
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...


