Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
Receptor-Mediated Endocytosis01:20

Receptor-Mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Naked antisense oligonucleotides remain endolysosomally sequestered despite induced membrane damage.

bioRxiv : the preprint server for biology·2026
Same author

How endosomal PIKfyve inhibition prevents viral membrane fusion and entry.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

SpatialDINO: A Self-Supervised 3D Vision Transformer that enables Segmentation and Tracking in Crowded Cellular Environments.

bioRxiv : the preprint server for biology·2026
Same author

How endosomal PIKfyve inhibition prevents viral membrane fusion and entry.

bioRxiv : the preprint server for biology·2025
Same author

Tau seeding in neurons enabled by transient endolysosomal perforations are confined within endolysosomes.

bioRxiv : the preprint server for biology·2025
Same author

Close-up of vesicular ER exit sites by volume electron imaging using FIB-SEM.

The Journal of cell biology·2025
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: Jun 19, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

Clathrinアダプターは,本当に適応しています.

Tom Kirchhausen1

  • 1Department of Cell Biology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA. kirchhausen@xta1200.harvard.edu

Cell
|June 28, 2002
PubMed
まとめ
この要約は機械生成です。

クラスリン経路は,内分泌と成長因子のダウンレギュレーションを媒介する. 研究者らは,アダプタータンパク質2 (AP-2) 核の原子構造を決定し,クラスリン層を貨物タンパク質に結合させる役割を示した.

さらに関連する動画

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
10:02

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Published on: October 23, 2016

関連する実験動画

Last Updated: Jun 19, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
10:02

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Published on: October 23, 2016

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学と構造生物学について

背景:

  • クラトリンの経路は,受容体媒介の内分細胞分裂と,成長因子信号伝達の調節において極めて重要です.
  • クラトリンアダプターは,クラトリンコートをトランスメブランの貨物タンパク質に接続し,ベジクルに分類します.

研究 の 目的:

  • アダプタタンパク質2 (AP-2) 核の原子構造を解明する.
  • AP-2が細胞膜と貨物タンパク質とどのように相互作用するかを理解する.

主な方法:

  • 原子構造を決定するためのX線結晶学または冷凍電子顕微鏡.
  • タンパク質とタンパク質の相互作用を研究するための生化学分析.

主要な成果:

  • AP-2核の原子構造が決定されました.
  • 構造は,AP-2と膜,そして貨物タンパク質の細胞溶融尾の間の特定の相互作用を明らかにします.

結論:

  • 解かれた構造は,クラスリン媒介性エンドサイトーシスの分子メカニズムに関する重要な洞察を提供します.
  • AP-2の構造を理解することで,受容体トラフィックと細胞信号伝達経路に関する研究が容易になります.