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Murine Nasal Lavage Fluid Collection without Blood Contamination05:12

Murine Nasal Lavage Fluid Collection without Blood Contamination

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The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory...
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OSTSS leads to pooled inflammatory mediators in the tear film, causing symptoms like epiphora and discomfort. Here, we present a case where therapeutic nasolacrimal lavage every 2 months resolved epiphora and itchiness, suggesting its efficacy as a therapeutic intervention for OSTSS. Additionally, we report subjective improvements in symptoms in 3 additional...
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Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
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A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental...
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Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment...
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A method to intranasally administer drugs to awake mice for the purpose of targeting the brain is described. This method allows for repeat dosing over long periods using intranasal administration of drug without anesthesia, and nose-to-brain delivery with minimal systemic...
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Updated: Jan 8, 2026

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
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E3ユビキチンリガゼは,糖鎖を認識する.

Yukiko Yoshida1, Tomoki Chiba, Fuminori Tokunaga

  • 1Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan.

Nature
|July 26, 2002
PubMed
まとめ

N-グリカンは,SCF (((Fbx2) ユビキチンリガゼ複合体を通じてタンパク質の分解をシグナルする. このメカニズムは,エンドプラズマの網膜 (ER) から転位したN-グリコシル化タンパク質を対象に分解し,タンパク質の品質管理に不可欠です.

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関連する実験動画

Last Updated: Jan 8, 2026

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科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学

背景:

  • エンドプラズマ網膜 (ER) のN-グリコシレーションは,タンパク質の品質管理に不可欠です.
  • タンパク質の分解経路は,細胞のプロテオスタシスを確保する.

研究 の 目的:

  • タンパク質の分解におけるN-グリカンの役割を調査する.
  • N-グリカン媒介による分解に関与する特定のユビキチンリガゼ複合体を特定する.

主な方法:

  • F-boxタンパク質Fbx2とN-グリコシル化タンパク質の相互作用を研究した.
  • F-boxドメイン (Fbx2 Delta F) を欠く変異Fbx2を用いて,分解経路への影響を評価した.
  • プロテアソーム阻害後のタンパク質の相互作用を調査した.

主要な成果:

  • N-グリカンは,SCF (((Fbx2) ユビキチンリガゼ複合体によって認識される分解信号として作用します.
  • Fbx2は,N結合の高マノースオリゴサッカライドを持つタンパク質に特異的に結合する.
  • プレインテグリンβ1は,シトソール内のFbx2と相互作用する.
  • 変異Fbx2デルタFは,ERに関連した分解基質の分解を抑制する.

結論:

  • SCF (((Fbx2) コンプレックスは,N-グリコシル化タンパク質をユビキチナートする.
  • このユビキチネーションは,タンパク質品質管理メカニズムによって媒介されるERからサイトゾールへの転位後に発生します.