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関連する概念動画

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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関連する実験動画

Updated: Jul 3, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

タンパク質のスプライシングは,小さな分子によって引き起こされます.

Henning D Mootz1, Tom W Muir

  • 1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.

Journal of the American Chemical Society
|August 1, 2002
PubMed
まとめ

研究者らは,小分子を使ってタンパク質の機能を制御する新しい方法を開発した. このテクニックは,タンパク質のスプライシングを活用し,ラパミシンに対する反応としてタンパク質の活性を時間的に正確に調節することができます.

科学分野:

  • 化学生物学 化学生物学とは
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • タンパク質の機能を小さな分子で制御することで,遺伝的方法では達成し難い時間的精度が得られます.
  • 現在の戦略では,タンパク質の活性部位に直接結合したり,構造的変化を誘導したりすることが多い.
  • タンパク質の主要な構造を小分子で変化させることで,代替的な制御メカニズムが提示されます.

研究 の 目的:

  • 小分子を使用してタンパク質の機能を制御するための新しい技術を導入する.
  • タンパク質の活性を誘導的に制御するために,タンパク質のスプライシングを活用する.
  • 小分子媒介タンパク質調節のための一般化可能な方法を開発する.

主な方法:

  • タンパク質のスプライシングを活用し,自然に起こる翻訳後のプロセスである.
  • 小分子ラパミシンによってタンパク質のスプライシングが誘発されるシステムを設計する.
  • 特定のN-およびC-エクステイン配列から独立した方法の開発.

主要な成果:

  • ラパミシンの存在下でのみタンパク質のスプライシングを可能にする技術を実証した.
  • 小分子誘発タンパク質の機能制御のための新しいメカニズムを確立しました.

さらに関連する動画

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

関連する実験動画

Last Updated: Jul 3, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

  • 異なるタンパク質ターゲットの間で広範な適用の可能性を示した.

    • 結論:

    • 開発された技術は,小分子でタンパク質の機能を制御するための一般化可能なプラットフォームを提供します.
    • このアプローチにより,タンパク質の活性が時間的に正確に調節されます.
    • この方法は,タンパク質操作のための新しいツールを導入することによって,化学遺伝学の分野を前進させます.