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関連する概念動画

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Structure of a Gene01:30

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

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関連する実験動画

Updated: Jun 25, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

コアプロモータークロマチンのアーキテクチャを変更することによって,遺伝子発現プログラムを変更する.

Stavros Lomvardas1, Dimitris Thanos

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.

Cell
|August 2, 2002
PubMed
まとめ

ウイルス感染症は,エンハンセオソーム組立とクロマチンの改造を通じてIFN-β遺伝子活性化を誘発する. キーヌクレオソームのプレポジショニングは,遺伝子発現のタイミングと特異性を変化させ,エンハンスオソームとクロマチンの構造の相互作用を強調します.

科学分野:

  • 分子生物学は分子生物学である.
  • 免疫学 免疫学とは
  • エピジェネティクス エピジェネティクス

背景:

  • インターフェロン-β (IFN-β) 遺伝子の転写活性化は,抗ウイルス反応に極めて重要です.
  • このプロセスは,エンハンセオソーム複合体の形成と,遺伝子のプロモーターにおけるダイナミックなクロマチンの改造を含む.
  • 重要なステップは,通常,コアプロモーターをブロックするヌクレオソームの位置変更です.

研究 の 目的:

  • IFN-β遺伝子発現プログラムの調節におけるヌクレオソームポジショニングの役割を調査する.
  • ヌクレオソームのプレポジショニングが,ウイルス感染に対する一時的および信号特異的な転写応答に影響するかどうかを決定する.

主な方法:

  • IFN-βプロモーターの核細胞位置の実験操作.
  • 変異性染色体構造の細胞におけるウイルスチャレンジ後の遺伝子発現パターンの分析.
  • 転写活性化運動と特異性の評価.

主要な成果:

  • IFN-βプロモーターを阻害するヌクレオソームを下流位置に先制的にスライドすることで,遺伝子の発現プロファイルが著しく変化しました.
  • 転写応答のタイミングとシグナル特異性は,標準的誘導経路と比較して顕著に変化しました.

さらに関連する動画

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

関連する実験動画

Last Updated: Jun 25, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

  • これは,初期クロマチンの構造が,その後の遺伝子発現プログラムに影響することを示している.

    • 結論:

    • 特定のエンハンスゾーム複合体とローカルクロマチンの構造の間のダイナミックな相互作用が,正確な遺伝子発現プログラムを決定する.
    • 特定の局所クロマチンの構造は,遺伝子発現プログラムのアイデンティティを確立し維持するために不可欠です.
    • この発見は,先天的な免疫反応を表遺伝子レベルで支配する規制メカニズムについての洞察を提供します.