ミューシン構造の原理: 合成グリコペプチドの構造研究で,モノ,ディ,トリ,ヘキササカリドグリコドメインを集積した
Don M Coltart1, Ajay K Royyuru, Lawrence J Williams
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
Journal of the American Chemical Society
|August 15, 2002
まとめ
NMRの研究は,CD43グリコペプチドのアルファ-O結合グリカンが定義された構造を作り出すことを明らかにしています. グライカンの表示に不可欠なこのコア構造は,ベータ結合によって影響を受けません.
科学分野:
- グライコバイオロジーは,
- 構造生物学 構造生物学とは
- バイオケミストリー バイオケミストリー
背景:
- 細胞表面グリコプロテインCD43は,細胞結合と免疫反応に役割を果たしています.
- ムシンのドメインはO-グリコシル化が強く,タンパク質の構造と機能に影響を与えます.
- グリコペプチドの構造を理解することは,それらの生物学的役割を解読する鍵です.
研究 の 目的:
- CD43.3からのムシンのグリコペプチドモチーフの構造特性を調査する.
- 異なるO-グリコシル化結合 (アルファ対β) がグリコペプチド構成に与える影響を決定する.
- 付着した炭水化物抗原の組織化におけるグリコペプチド核の役割を明らかにする.
主な方法:
- 様々なCD43グリコペプチド構造物の全化学合成.
- 構造分析のための核磁気共振 (NMR) スペクトロスコーピー.
- 3D構造を解くための制限された分子ダイナミクス計算.
主要な成果:
- アルファ-O-リンクされたグリコペプチドの定義されたグリコペプチド核構成が特定されました.
- このコア構造は,ペプチドと最初のN-アセチルガラクトサミン残基の相互作用によって確立されます.
- アルファ-O-グリコシライゼーションはペプチドの骨格に顕著な影響を及ぼし,ベータ結合は最小限の影響を及ぼします.
- コア構造は,多様なグリカン表示のための共通の枠組みとして機能します.
結論:
- O-グリコシライゼーション結合のモードは,グリコペプチド構造を決定的に決定する.
- アルファ-O-リンクされたグリカンは,CD43グリコペプチドの骨格に特定の形状を課します.
- グリコペプチドの核構造は,多様な炭水化物エピトープの提示に不可欠です.
関連する概念動画
01:44What are Carbohydrates?
Overview
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
02:15Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
01:28Structures of Carboxylic Acid Derivatives
Structure of Carboxylic Acid Derivatives
Carboxylic acid derivatives contain an acyl group attached to a heteroatom such as chlorine, oxygen, or nitrogen. The carbonyl carbon and oxygen are both sp2-hybridized with an unhybridized p orbital.
The three sp2 orbitals of the carbonyl carbon form three σ bonds, one each with the carbonyl oxygen, the α carbon, and the heteroatom, whereas the other two sp2 orbitals of the carbonyl oxygen are occupied by the lone pairs. Further, the unhybridized p...
Carboxylic acid derivatives contain an acyl group attached to a heteroatom such as chlorine, oxygen, or nitrogen. The carbonyl carbon and oxygen are both sp2-hybridized with an unhybridized p orbital.
The three sp2 orbitals of the carbonyl carbon form three σ bonds, one each with the carbonyl oxygen, the α carbon, and the heteroatom, whereas the other two sp2 orbitals of the carbonyl oxygen are occupied by the lone pairs. Further, the unhybridized p...
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...