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Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Published on: May 3, 2013
滑らかな筋肉の原始細胞は,人間の血液の原始細胞である
David Simper1, Paul G Stalboerger, Carmelo J Panetta
1Division of Cardiovascular Diseases and Molecular Medicine Program, Mayo Clinic, Rochester, Minn 55905, USA.
Circulation
|September 5, 2002
まとめ
研究者は,ヒトの血液から滑らかな筋肉の原始細胞 (SPC) を培養し,特定のインテグリンマーカーと粘着性の増加を特定しました. この発見は,成人における血管の滑らかな筋肉細胞の分化とホーミングの理解を前進させる.
科学分野:
- 心血管生物学 心血管生物学
- 幹細胞の研究について
- 細胞および分子医学は,細胞および分子医学である.
背景:
- 動物の研究は,骨髄由来細胞がネオインティマの血管性滑らかな筋肉を形成することを示唆しており,循環する滑らかな筋肉の原始細胞 (SPC) を示唆しています.
- ヒトの循環中のSPCの証拠は不足しており,プラーク部位への帰着メカニズム (潜在的にインテグリンを含む) は理解されていません.
研究 の 目的:
- 人間の外周血液SPCから,滑らかな筋肉の増殖細胞 (SOC) を培養する.
- これらのSOCの表面積インテグリン表現を特徴付けるために.
主な方法:
- 人間の単核細胞を分離し,コラーゲン1型に培養した.
- 増殖細胞は,血小板由来成長因子BB (PDGF-BB) を含むまたは含まない,内皮成長媒介 (EGM-2) を使用して選択されました.
- 細胞マーカーとインテグリン発現を分析するために,免疫光,ウエスタン・ブロッティング,フローサイトメトリ (FACS) が使用されました.
主要な成果:
- PDGF-BBを濃縮した媒体は,SOCの急速な脱出と拡大を促した (>40の人口倍増).
- SOCは,滑らかな筋肉特有のマーカー (アルファSMA,ミオシン,カルポニン) と血管新生マーカー (CD34,Flt1,Flk1) を表現した.
- SOCはインテグリンα5β1発現が著しく増加し,内皮外成長細胞 (EOC) に比べてフィブロネクチンへの粘着が8倍強かった.
結論:
- この研究は,推定の人間のSPCから滑らかな筋肉細胞の成長が成功していることを示しています.
- これらの細胞は,独特の成長,粘着,インテグリンプロファイルを示しており,血管修復の可能性を示唆しています.
- この発見は,成人血管の滑らかな筋肉細胞の分化,増殖,帰着メカニズムの理解に寄与する.
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