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関連する概念動画

Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Structure of Cardiac Muscles01:13

Structure of Cardiac Muscles

Cardiac muscle, or myocardium, is a specialized type of muscle found exclusively in the heart. Its unique structural and functional characteristics enable the heart to perform its vital role of pumping blood throughout the body continuously and rhythmically. The cardiac muscle cells, or cardiomyocytes, possess an endomysium and perimysium but do not have an epimysium.
Compared to skeletal muscles, cardiac muscle cells are small and mostly have a single nucleus. Additionally, they are usually...
Specialized Characteristics of Cardiac Muscles01:27

Specialized Characteristics of Cardiac Muscles

The primary role of cardiac muscles is to propel blood throughout the cardiovascular system. The cardiac muscle cells, or cardiomyocytes, exhibit specialized characteristics that allow them to perform this function.
Cardiac muscle cells are smaller than skeletal muscles, averaging 10–20 mm in diameter and 50–100 mm in length. However, they have large energy demands for continuous contraction and relaxation. This energy is almost exclusively derived from aerobic metabolism of energy reserves in...

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関連する実験動画

Updated: May 13, 2026

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:46

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

幹細胞に由来する心筋筋細胞は,不律性潜在的な発作を示しています.

Ying Ming Zhang1, Criss Hartzell, Michael Narlow

  • 1Department of Medicine, Emory University, Atlanta, Ga 30033, USA.

Circulation
|September 5, 2002
PubMed
まとめ

胚性幹細胞 (ESC) と胚性癌細胞 (ECC) は,心筋細胞に微分化され,自発的な活動や誘発性心律不全を含むリズムを誘発する性質を示し,細胞療法での使用には注意が必要です.

科学分野:

  • 心臓病学 心臓病学
  • 幹細胞生物学 幹細胞生物学
  • 電気生理学 電気生理学

背景:

  • 胚性幹細胞 (ESC) や胚性癌細胞 (ECC) から派生した心筋細胞 (CM) は,成人の肌細胞の特徴の一部を備えているが,すべてではない.
  • ESCは,損傷した心筋膜に移植の可能性を示しており,心筋病症の治療用途を示唆しています.
  • ESCおよびECC由来CMのリズム不振性特性は,徹底的な調査を必要としています.

研究 の 目的:

  • マウスのESCおよびECCから微分化された心筋細胞のリズム障害を引き起こす可能性を調査する.
  • これらの派生CMにおける電気生理学的性質を特徴づけ,潜在的不律性メカニズムを特定する.

主な方法:

  • 全細胞パッチクランプの記録は,心筋細胞を in vitro で研究するために使用されました.
  • アクションポテンシャル (AP) 形態学,アップストローク速度 (dV/dt),AP持続時間などを分析した.
  • 薬剤およびイオン操作を用い,誘発された活性とデポラライゼーション後の活性を誘発および評価した.

主要な成果:

  • 派生CMは,AP形態の有意な異質性,最大dV/dtの減少,およびAPの持続時間の延長を示した.
  • 培養CMにおいて,自発的な電気活動と頻繁に誘発された活動が観察されました.

さらに関連する動画

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
14:03

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

関連する実験動画

Last Updated: May 13, 2026

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:46

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
14:03

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

  • 初期アフターデポラライゼーション (EAD) は,薬理学的刺激やイオン変化を含む様々な条件下で誘導可能であった.
  • デポラライゼーション後の遅延 (DADs) は,高カルセミアまたはイソプロテレノールによって容易に誘発された.

    • 結論:

    • ESCとECCに由来するCMは,少なくとも3つの異なるAP現象型に分類されます.
    • これらのCMは,自発的な活動,dV/dtの障害,APの長期間,および容易に誘発可能なトリガー型不律症を示します.
    • これらの発見は,細胞移植療法におけるESCの使用に関して,再入場,自動性,またはトリガーされた活動を通じて不律を誘発する可能性があるため,注意を払う必要がある.