嘔吐鼻受容体遺伝子のクラスタが欠けているマウスのフェロモン反応が欠けている
Karina Del Punta1, Trese Leinders-Zufall, Ivan Rodriguez
1The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Nature
|September 6, 2002
まとめ
嘔吐鼻臓器 (VNO) はフェロモンを検出するが,その受容体は不明であった. マウスのV1r遺伝子を削除すると,フェロモンの検出が低下し,社会的行動が変化し,V1rが確認されました.
科学分野:
- 神経科学は神経科学である.
- 遺伝学 遺伝学とは
- 動物の行動 動物の行動
背景:
- 哺乳類の嘔吐鼻臓器 (VNO) はフェロモンを検知し,社会的および生殖行動に不可欠である.
- VNOにおけるフェロモンの検出に責任を負う分子受容体は,ほとんど未定義のままである.
- 候補フェロモン受容体はV1rおよびV2r遺伝子のスーパーファミリーによってコードされ,VNO感覚ニューロンで発現します.
研究 の 目的:
- フェロモン検出とVNO依存行動におけるV1r遺伝子の機能的役割を調査する.
- フェロモン受容体としてのV1r受容体の機能的証拠を提供するため.
主な方法:
- クロモソーム工学を使って,マウスのV1r遺伝子を無傷に16個含む600キロベースゲノム領域を削除しました.
- ミュータントマウスの行動の変化を評価し,男性の性行為と母親の攻撃性に焦点を当てました.
- フェロモンリガンドに対する反応を評価するために,VNO表皮の電気生理学的記録を行った.
主要な成果:
- ミュータントマウスは,変異した男性性行動や母性攻撃性を含む,特定のVNOに依存する行動において重大な欠陥を示した.
- 電気生理学的分析では,変異したマウスのVNO表皮に特定のフェロモンリガンドに対する検出可能な反応は示されなかった.
- 削除は,V1r遺伝子レパートリーの約12%に影響を与え,V1r遺伝子ファミリーを2つ含む.
結論:
- V1r欠乏マウスにおける観察された行動障害と化学感覚的欠陥は,フェロモン検出におけるV1r受容体の役割を強く支持しています.
- この研究は,V1r遺伝子をフェロモン反応とVNO媒介の行動と結びつける最初の機能的証拠を提供します.
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