ヒトのスプライソームの包括的なタンパク質学的分析
Zhaolan Zhou1, Lawrence J Licklider, Steven P Gygi
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Nature
|September 13, 2002
まとめ
研究者らは,遺伝子発現に不可欠な複雑な細胞機械であるスプライセソームに約145のタンパク質を特定しました. この包括的なタンパク質の在庫は,新しい成分を明らかにし,遺伝子発現のステップを調整するスプライソームの役割を強調しています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- スプライセソームは,前メッセンジャーRNAからイントロンを除去する大きな分子マシンです.
- 以前の研究では,精製されたサブコンプレックスを使用してスプライソームの構成要素を特徴づけましたが,完ぺきなマシンの完全な在庫は,難解でした.
研究 の 目的:
- 機能性スプライソソームの完全なタンパク質組成を特定する.
- スプライセソームタンパク質の複雑性と潜在的な規制的役割を特徴付ける.
主な方法:
- マルトース結合タンパク質親和性染色体を用いて,無傷で機能的なスプライソームを分離する.
- ナノスケールマイクロキャピラリ液体染色学タンデム質量スペクトロメトリーによる分離されたスプライソームのプロテオミック分析.
主要な成果:
- 約145種類の異なるスプライソソームタンパク質を特定し,これまでに特徴づけられた中で最も複雑な細胞機械として確立しました.
- 以前に知られているすべてのスプライシング因子の確認と58の新成分の発見.
- 少なくとも30の特定されたタンパク質が,スプライシング以外にも役割を持っていることを発見し,より広範な遺伝子発現機能を示唆しています.
結論:
- スプライソームは高度に複雑な機械で,タンパク質のレパートリーはこれまで考えられていたよりもはるかに大きい.
- スプライソーム内の広範なタンパク質ネットワークは,おそらく,他の遺伝子発現プロセスとのプレメッセンジャーRNAスプライシングの複雑な調節と結合を容易にする.
関連する概念動画
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
01:05Single Nucleotide Polymorphisms-SNPs
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
02:41Chromatin Structure and RNA Splicing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...