アデノウイルス繊維は,CAR媒介の細胞間粘着を阻害し,ウイルスの脱出を可能にします
Robert W Walters1, Paul Freimuth, Thomas O Moninger
1Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA.
Cell
|September 26, 2002
まとめ
アデノウイルスは,その受容体であるコクサッキー・アデノウイルス受容体 (CAR) を細胞への侵入に使用し,その後CARを再利用して上皮の障壁を破壊し,新しい宿主感染のために環境への放出を可能にします.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 細胞生物学 細胞生物学
- 皮質生物学 エピテリア生物学
背景:
- アデノウイルスは,細胞に感染し,複製する一般的なヒト病原体です.
- ウイルスの効率的な拡散には,感染した細胞から出て新しい宿主に拡散するメカニズムが必要です.
- 表面膜の壁は,病原体の退去に重大な課題をもたらします.
研究 の 目的:
- ヒトの呼吸道上皮質からアデノウイルスの脱出のメカニズムを調査する.
- ウイルス拡散におけるアデノウイルス繊維タンパク質とその受容体 (CAR) の役割を決定する.
- アデノウイルスが表皮の障壁を克服する方法を理解するために.
主な方法:
- アデノウイルスによるヒト呼吸道上皮細胞培養物の感染.
- ウイルスの放出パターンの分析 (アピカル対ベースラテラル).
- アデノウイルス繊維タンパク質とコクサッキアデノウイルス受容体 (CAR) 相互作用の役割の調査.
主要な成果:
- アデノウイルスは当初,呼吸道上皮質の底側表面に放出された.
- その後,ウイルスは上皮細胞の間を通り抜け,頂上表面に現れる.
- アデノウイルスの繊維タンパク質が基礎側CARに結合すると,緊密な結合が破壊され,パラセルラーウイルスの脱出が容易になりました.
結論:
- アデノウイルスは,その受容体であるCARを細胞への初期侵入と,その後の退出の両方に利用します.
- 繊維タンパク質とCARの相互作用は,上皮の緊密な接点を破壊し,頂点の放出を可能にするために重要です.
- CARのこの二重利用は,上皮の障壁を越えてアデノウイルス拡散のための洗練された戦略を強調しています.
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