Dec1とDec2は,哺乳類の分子時計の調節体である
Sato Honma1, Takeshi Kawamoto, Yumiko Takagi
1Department of Physiology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
Nature
|October 25, 2002
まとめ
哺乳類の昼夜リズムは,時計遺伝子が調節しています. 新しい研究は,Dec1とDec2を重要な調節体として特定し,分子時計に影響を与える第5の時計遺伝子ファミリーを形成しています.
科学分野:
- クロノバイオロジーはクロノバイオロジーを用います.
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- 哺乳類の昼夜リズムは,上神核によって制御される.
- 4つの既知の時計遺伝子ファミリーは,細胞内リズム生成のための転写-翻訳フィードバックループを形成する.
- CLOCKおよびBMAL1タンパク質はPER遺伝子転写を駆動し,PERおよびCRYタンパク質によって抑制されます.
研究 の 目的:
- 哺乳類の分子時計におけるDec1とDec2の役割を調査する.
- Dec1とDec2が既知の時計遺伝子成分と相互作用するかどうかを判断する.
- 上核におけるDec1とDec2の発現パターンと調節を特徴づける.
主な方法:
- マウスのPer1プロモーター活性に対するDec1とDec2の効果を調査した.
- Dec1/Dec2 と CLOCK/BMAL1.1 の間のタンパク質とタンパク質の相互作用を分析した.
- 視床上核におけるDec1とDec2の昼夜表現と光による変化を調べた.
主要な成果:
- Dec1とDec2は,Per1プロモーターのCLOCK/BMAL1媒介トランザクティベーションを抑制する.
- 抑制は,BMAL1との直接的な相互作用および/またはE-box要素の競争を通じて発生します.
- Dec1とDec2は,スプラキアスマティック核で昼間の発現を示し,主観的な昼間にピークに達します.
- 光パルスは,相依存の方法でDec1発現を誘導する.
結論:
- Dec1とDec2は,哺乳類の分子時計の中で抑制剤として機能します.
- これらのタンパク質は,新しい第5の時計遺伝子の家族を表しています.
- Dec1とDec2は,哺乳類の昼夜リズムを整合的に調節する.
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