Saccharomyces cerevisiaeにおけるトランスクリプション制御ネットワーク

Tong Ihn Lee¹, Nicola J Rinaldi, François Robert

¹Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)

|October 26, 2002

まとめ

イーストの転写レギュレータが,生きている細胞の遺伝子とどのように相互作用するかをマッピングしました. これは,細胞機能と遺伝子発現プログラムに不可欠な複雑な規制ネットワークを明らかにします.

科学分野:

分子生物学は分子生物学である.
システム生物学システム生物学
ゲノミクスゲノミクスとは

背景:

遺伝子調節を理解することは,細胞機能の解読に不可欠です.
転写調節体は,遺伝子発現を制御する上で重要な役割を果たします.
これらの相互作用をマッピングすることで,細胞ネットワークの洞察が得られます.

研究の目的:

転写レギュレータがSaccharomyces cerevisiaeの遺伝子とどのように関連するのかを決定する.
酵母における調節遺伝子相互作用のネットワークを構築する.
ネットワークのモチーフと,規制構造の組み立てにおけるその役割を特定する.

主な方法:

生きた酵母細胞における転写調節基因結合の全ゲノム分析.
ネットワークモチーフの識別,ネットワークアーキテクチャの基本単位.
モチーフを用いた転写制御ネットワークを組み立てるための自動化されたプロセスの開発.

主要な成果:

Saccharomyces cerevisiaeのほとんどの転写レギュレータ-遺伝子関連性の包括的な地図が作成されました.
ネットワークモチーフは,転写制御ネットワークの重要な構成要素として特定されました.
自動化された方法により,これらのモチーフから規制ネットワークを構築する可能性を実証しました.

結論:

ユカリオット細胞の機能は,広範囲にわたる転写レギュレータのネットワークを通じて相互接続されています.
これらのネットワークには,他の規制当局を制御する規制当局が関与し,複雑な規制階層を形成します.
この発見は,酵母における世界的な遺伝子発現調節を理解するための枠組みを提供する.

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