翼のないドロソフィラWntは,シナプス前とシナプス後の分化のための重要な信号を提供します
Mary Packard1, Ellen Sumin Koo, Michael Gorczyca
1Department of Biology, University of Massachusetts, Amherst, MA 01003, USA.
Cell
|November 7, 2002
まとめ
ドロソフィラ・ウィングレス (Wg) タンパク質はシナプスの発達に不可欠です. Wgの喪失はシナプス形成を妨害し,グルタマタージック神経筋肉の交差点における異常な前および後シナプス構造につながります.
科学分野:
- 神経科学は神経科学である.
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
背景:
- アグリンは脊椎動物の神経筋結節 (NMJ) の発達に不可欠です.
- 中枢シナプス形成を調節する分子は,ほとんど未知のままである.
- Wntタンパク質は,モルフォゲンとして知られており,シナプスの成熟に関与しています.
研究 の 目的:
- シナプスの発達におけるWnt家族の一員であるDrosophila Wingless (Wg) の役割を調査する.
- Wgがグルタマタージックシナプスの前・後シナプス区画の形成に関与するかどうかを判断する.
主な方法:
- グルタマタージックNMJにおけるWgとその受容体の発現分析.
- シナプス形成に対するWg喪失の影響を評価するための遺伝子操作.
- シナプスボタン発達の顕微鏡検査,活性ゾーン,そしてシナプス後の専門化.
主要な成果:
- Wgとその受容体は,ドロソフィラのグルタマタージ性NMJで発現する.
- Wgはシナプス突起によって分泌されます.
- Wg機能の喪失は,ターゲット依存のシナプス形成を大幅に減少させます.
- Wgが欠けているシナプス突起は,アクティブゾーンとポストシナプス専門性の発達に失敗または異常を示します.
結論:
- ドロソフィラ・ウィングレス (Wg) はシナプスの発達に不可欠です.
- Wgシグナリングは,シナプス前とシナプス後のコンパートメントの両方の協調的な発達に不可欠です.
- Wgは,グルタマテルギー性NMJにおけるシナプス形成の重要な調節体として作用する.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...