SRタンパク質SRp38は,M相細胞のスプライシングを抑制する
Chanseok Shin1, James L Manley
1Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Cell
|November 7, 2002
まとめ
SRタンパク質は遺伝子発現を調節する. 特定のSRタンパク質であるSRp38は,特に細胞分裂中に脱酸化されるとき,スプライシング抑制剤として作用し,遺伝子発現制御に影響を与えます.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝子規制 遺伝子規制
背景:
- SRタンパク質は,mRNA前スプライシングにおいて極めて重要であり,構成的および調節されたスプライシング経路の両方に影響を与えます.
- SRタンパク質ファミリーは,遺伝子発現の調節において重要な役割を果たします.
研究 の 目的:
- 新型SRタンパク質SRp38の特徴を明らかにし,スプライシングにおけるその機能を明らかにする.
- 細胞サイクル制御におけるSRp38の役割とその遺伝子発現への影響を調査する.
主な方法:
- SRp38.8の活性を評価するために,スプライシングアッセイが行われました.
- 異なる細胞サイクル段階の細胞抽出物は,SRp38のリン酸化状態を分析した.
- スプライシング阻害に対する非酸化SRp38の効果を調べました.
主要な成果:
- SRp38は,標準的なスプライシングアクティベーションアッセイでは無効であるため,ユニークな性質を現しています.
- デフォスフォリレーションは,SRp38を強力なスプライシング・レプレッサーに変換し,早期にスプライシングを抑制します.
- SRp38はミトーシス細胞で特異的に脱酸化され,ミトーシス中のスプライシング阻害と相関しています.
結論:
- 脱酸化SRp38は,ミトーシス中のスプライシング抑制の重要な調節体である.
- SRp38は,スプライシングの調節を通じて,細胞サイクル中の遺伝子発現を制御する上で重要な役割を果たします.
関連する概念動画
Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Directing Proteins to the Rough Endoplasmic Reticulum
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...


