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関連する概念動画

Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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関連する実験動画

Updated: Jul 19, 2026

Recombinant &#945;- &#946;- and &#947;-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

NPAS2:ガス反応性トランスクリプション因子

Elhadji M Dioum1, Jared Rutter, Jason R Tuckerman

  • 1Departments of Biochemistry and Plant Biology and Plant Biotechnology Center, The Ohio State University, 1060 Carmack Road, Columbus, OH 43210, USA.

Science (New York, N.Y.)
|November 26, 2002
PubMed
まとめ

ニューロンのPASドメインタンパク質2 (NPAS2) は,DNA結合を調節する分子であるヘムと結合する. 一酸化炭素はNPAS2-BMAL1ヘテロダイマーを破壊し,日中リズム遺伝子発現に影響を与えます.

科学分野:

  • 分子生物学は分子生物学である.
  • クロノバイオロジーはクロノバイオロジーを用います.
  • バイオケミストリー バイオケミストリー

背景:

  • ニューロンのPASドメインタンパク質2 (NPAS2) は,日中リズム調節に不可欠な転写因子です.
  • NPAS2はBMAL1とのヘテロダイマーとして機能し,遺伝子発現を制御するためにDNAを結合します.

研究 の 目的:

  • NPAS2の機能におけるヘムの役割を調査する.
  • ヘム状態とガス状分子がNPAS2-BMAL1のDNA結合と昼夜調節に影響を与えるメカニズムを解明する.

主な方法:

  • In vitro DNA結合アッセイは,NPAS2-BMAL1ヘテロダイマーを使用して,アポ (ヘムフリー) およびホロ (ヘム負荷) 状態で行われました.
  • 異なる割合のNADP (H) と一酸化炭素 (CO) がDNA結合に与える影響を評価した.

主要な成果:

  • NPAS2の両方のPASドメインは,DNA結合活動を調節するヘムと結合する.
  • ヘム負荷のNPAS2-BMAL1ヘテロダイマーは,特定の還元条件下で活発なDNA結合を示した.
  • 一酸化炭素は,Holo-NPAS2のDNA結合を阻害し,BMAL1のホモジマー形成を促進し,NPAS2-BMAL1のヘテロジマーを破壊した.

さらに関連する動画

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

関連する実験動画

Last Updated: Jul 19, 2026

Recombinant &#945;- &#946;- and &#947;-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

結論:

  • ヘミはNPAS2における義肢グループとして作用し,細胞の酸化還元とガス信号を感知し,統合する.
  • NPAS2-BMAL1の異体化およびその後の遺伝子調節は,ヘムベースのガスセンシングによって制御され,日中リズムのための新しい調節メカニズムを提供します.