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Updated: Jul 6, 2026

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

単細胞における膜タンパク質の横向拡散の測定

M Edidin, Y Zagyansky, T J Lardner

    Science (New York, N.Y.)
    |February 6, 1976
    PubMed
    まとめ

    培養細胞におけるレーザー漂白後の光回復は,統合膜タンパク質の拡散を測定する. この技術は,細胞膜の表面にラベルを貼ったタンパク質の横向移動を定量化します.

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    Protein Diffusion in the Membrane01:24

    Protein Diffusion in the Membrane

    Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...