ヒトゲノムのタンパク質キナーゼ補完体である
G Manning1, D B Whyte, R Martinez
1SUGEN Inc., 230 East Grand Avenue, South San Francisco, CA 94080, USA. gerard-manning@sugen.com
まとめ
研究者はヒトのタンパク質キナーゼ補完体 (キノーム) を分類し,518の遺伝子を特定し,そのうち71の新規キナーゼが含まれています. このヒトキノーム解析は,健康と病気におけるタンパク質のリン酸化を研究するための基礎を提供します.
科学分野:
- ゲノミクスゲノミクスとは
- 分子生物学は分子生物学である.
- バイオインフォマティックス
背景:
- 人間のゲノムには膨大な数のタンパク質キナーゼ遺伝子が含まれており,細胞シグナル伝達に不可欠です.
- これらの遺伝子 (キノーム) の完全なセットを理解することは,様々な生物学的文脈でタンパク質のリン酸化を分析するために不可欠です.
研究 の 目的:
- ヒトのタンパク質キナーゼを包括的にカタログ化する.
- 新種のタンパク質キナーゼ遺伝子を特定し,既存の配列を精製する.
- タンパク質のリン酸化を正常状態と疾患状態で分析するための基礎を提供すること.
主な方法:
- 公的および特許のゲノム,補完DNA (cDNA),および表現シーケンスタグ (EST) シーケンスを使用しました.
- モデル生物キノームとの比較分析を行った.
- 特定されたキナーゼ遺伝子の染色体マッピングを行いました.
主要な成果:
- 518の推定ヒトタンパク質キナーゼ遺伝子を特定し,これまでに報告されていない71のキナーゼを含む.
- 56の既知のキナーゼのタンパク質配列を拡張または修正した.
- 106のタンパク質キナーゼ擬似遺伝子を発見し,244のキナーゼを疾患ロシまたはがんアンプリコンにマッピングしました.
結論:
- カタログ化されたヒトキノームは,将来の研究のための包括的なリソースを提供します.
- 新しいキナーゼと保存されたファミリーが特定され,キノームの理解を広げました.
- キナーゼ遺伝子の分布は,ヒト特異的な進化と疾患における潜在的な役割を示唆しています.
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