Srb10/Cdk8は,転写因子Ste12をリン酸化することによって,酵母菌の糸状成長を調節する
Chris Nelson1, Susan Goto, Karen Lund
1Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Nature
|January 10, 2003
まとめ
サイクリン依存キナーゼSrb10は,Ste12をリン酸化することによって,酵母糸状の成長を調節する. 栄養素の制限によりSrb10の活性が低下し,Saccharomyces cerevisiaeにおけるSte12の機能とシドオヒファルの発達を促進する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
背景:
- 芽生える酵母Saccharomyces cerevisiaeは,栄養素の制限下で繊維状の成長を示します.
- この差別化は,Ste12転写因子とMAPキナーゼカスケードに依存しています.
- 栄養素制限によるSte12の調節メカニズムは,以前は定義されていなかった.
研究 の 目的:
- 栄養に制限された繊維の成長中にSte12機能を調節するメカニズムを解明する.
- この過程におけるサイクリン依存キナーゼSrb10の役割を特定する.
主な方法:
- Srb10とSte12の相互作用を調査しました.
- Srb10のキナーゼ活性と,Ste12のリン酸化と安定性に対する効果を分析した.
- 糸状の成長とフェロモン反応への影響を評価するために,遺伝子変異を利用した.
主要な成果:
- Srb10 (Cdk8) は,富裕な介質で Ste12 をリン酸化し,不安定化させることで,糸状の成長を阻害する.
- 窒素の制限により,Srb10のタンパク質とキナーゼの活性が低下し,Ste12のリン酸化が低下する.
- Srb10に依存するリン酸化部位の変異は,フェロモン反応に影響を与えることなく,擬似頭蓋骨の発達を高めます.
結論:
- Srb10は,窒素制限への反応として,糸状の成長のためのSte12活動の主要な調節剤として作用します.
- Srb10キナーゼの活動は,交配フェロモン経路とは独立して調節されます.
- Srb10は,生理学的信号に基づく転写因子を調節することによって,遺伝子発現と成長可能性を調整します.
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