1マイクロから1ミリ秒の時間範囲でDNAの動態を反映したDNAのピレン二元基離子形成
Kiyohiko Kawai1, Kei Miyamoto, Sachiko Tojo
1The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. kiyohiko@sanken.osaka-u.ac.jp
Journal of the American Chemical Society
|January 23, 2003
まとめ
二重ピレン結合オリゴデオキシヌクレオチド (ODN) は,DNAのダイナミクスを明らかにする. ピレン二元根カチオン (Py2(.+)) の形成速度は,電荷共振相互作用を通じて,一時的なDNA構造の洞察を提供します.
科学分野:
- 生物物理化学 生物物理化学
- 分子生物学は分子生物学である.
- スペクトル顕微鏡検査です.
背景:
- ピレン (Py) と結合したオリゴデオキシヌクレオチド (ODN) は,ユニークなスペクトル学的性質を備えています.
- DNAのダイナミクスを理解することは,様々な生物学的プロセスと治療上の応用において極めて重要です.
研究 の 目的:
- DNAダイナミクスを研究するために,二重ピレン結合ODNを合成する.
- パルス放射解析を用いて,ピレンジーマーラジカルカチオン (Py2(.+)) の形成速度を測定する.
- Py2(.+) 形成の動態と一時的なDNAの構造動態を相関させるため.
主な方法:
- 二重ピレン結合オリゴデオキシヌクレオチド (ODN) の合成.
- ODN溶液のD2Oのパルス放射分解は,K2S2O8.8.でK2S2O8.8.でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8でK2S2O8.8で
- 470nmでのピレン根カチオン (Py(.+)) と1500nmでのPy2(.+) (電荷共振帯) のスペクトルモニタリング.
主要な成果:
- Pyの形成は,<5マイクロ秒で観察されました.
- Py (((.+) の同時分解と Py2 (((.+) の形成は,約100マイクロ秒で観察されました.
- Py2(.+) の形成速度は,DNAの動態を直接反映し,電荷共鳴 (CR) 相互作用によって相互作用するPy(.+) 種を捕まえる.
結論:
- 特徴的な近赤外線CR吸収帯を持つPy2 ((+)) の形成率は,貴重なツールです.
- この方法は,一時的に形成されたDNA構造の構造的および動的情報を提供します.
- この研究は,1マイクロ秒から1ミリ秒までの時間帯をカバーしています.
さらに関連する動画
関連する概念動画
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The DNA Helix
Overview
Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
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